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从受污染的土壤中分离高产聚酸酸生产细菌,并对生物聚合物进行表征

Maria Abdul Salam¹, Arsalan Hussain², Uzma³

¹College of Agriculture Engineering and Food Science, Shandong University of Technology, Zibo, 255000, China. maria.abdulsalam@mlt.iiui.edu.pk.

Scientific reports

|November 23, 2025

概括

研究人员从受污染的土壤中分离出产生高产多基酸盐 (PHAs) 的细菌. 在优化条件下,PHA的产量达到70.44%,证实了其作为可生物降解塑料替代品的潜力.

科学领域:

微生物学微生物学
生物技术是生物技术.
聚合物科学聚合物科学

背景情况:

合成塑料由于其持久性和外来生物释放,给环境带来了重大挑战.
聚酸酸 (PHAs) 是微生物产生的可生物降解生物聚合物,为传统塑料提供了可持续的替代品.
隔离产生PHA的新型菌株对于开发高效和成本效益的生物聚合物生产至关重要.

研究的目的:

从受石油污染的土壤中分离和描述高产量的PHA产生细菌菌株.
优化培养条件以最大限度地提高PHA生产.
为了确认提取的生物聚合物的组成和结构.

主要方法:

从受石油污染的土壤样本中分离细菌.
16S rRNA测序用于细菌识别.
苏丹黑B染色用于PHA颗粒检测.
优化培养参数 (pH,温度,碳/来源,化时间).
使用二甲基硫酸盐 (SDS) 沉提取PHA.
使用福利埃变换红外光谱 (FTIR) 和质子核磁共振 (1H NMR) 的结构分析.

主要成果:

鉴定了五种细菌菌株,包括Paenibacillus lautus,Alcaligenes pakistanensis,Acinetobacter baumannii,Bacillus cereus和Bacillus tropicus,这些细菌菌株被发现.
优化条件 (pH 7.0,35-40°C,葡萄糖和) 导致PHA的峰值产量在60-70小时内达到70.44%±0.08%的干细胞重量.
通过FTIR和1HNMR分析证实了聚基酸 (PHB) 和聚基酸-co-3-hydroxyvalerate (PHBV) 共聚物的存在.

结论:

孤立的细菌菌株能够产生高产量的PHA.
优化的发酵条件显著提高PHA积累.
鉴定到的PHAs具有结构特征,适用于生物医学,生物技术和可持续制造领域的各种应用.

关键词:

申请申请表申请表细菌杆菌是一种生物塑料是生物塑料.生物聚合物是一种生物聚合物.聚酸酸多基甲酸

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