患有炎症性肠病的患者对HCoV-NL63的敏感性增加
Tingting Ning1, Nianchen Liu1, Qi Jiang2
1Department of Gastroenterology, Beijing Friendship Hospital, National Clinical Research Center for Digestive Disease, Beijing Digestive Disease Center, State Key Laboratory of Digestive Health, Capital Medical University, Beijing, 100050, No. 95 Yongan Street, Xicheng District, China.
炎症性肠病 (IBD) 患者由于ACE2表达率较高和中和抗体水平较低,对人类冠状病毒NL63 (HCoV-NL63) 感染的敏感性增加. 建议IBD患者采取额外的预防措施,以防止HCoV-NL63感染.
科学领域:
- 胃肠病学 胃肠病学
- 病毒学 病毒学
- 免疫学 免疫学 免疫学
背景情况:
- 免疫功能低下的炎症性肠病 (IBD) 患者面临严重呼吸道感染的风险,包括肺炎和支气管炎,来自人类冠状病毒NL63 (HCoV-NL63).
- 对于IBD患者对HCoV-NL63感染的易感性仍然不太清楚,特别是在结肠中ACE2受体的作用方面.
研究的目的:
- 调查IBD患者对HCoV-NL63感染的易感性.
- 检查IBD患者结肠粘膜中的 ангиотензин转化酶2 (ACE2) 的表达及其在HCoV-NL63感染中的作用.
- 与健康对照组相比,评估IBD患者对HCoV-NL63的中和抗体水平.
主要方法:
- 在各种组织和细胞系中ACE2表达的比较,包括IBD患者和动物模型的结肠组织.
- 使用基于伪病毒的系统评估结肠细胞对HCoV-NL63的敏感性.
- 在IBD患者和对照群中通过基于伪病毒的中和试验量化对HCoV-NL63的中和抗体.
主要成果:
- 在结肠组织和细胞中,ACE2的表达很高,这些组织和细胞对HCoV-NL63.3敏感.
- 在IBD患者,动物模型和炎症性结肠细胞的结肠粘膜中,ACE2表达升高,增加了它们对HCoV-NL63.3的易感性.
- IBD患者,特别是男性和老年人,表现出对HCoV-NL63的保护性中和抗体的度降低.
结论:
- 由于ACE2表达增加和中和抗体反应减少,IBD患者对HCoV-NL63感染的敏感性增加.
- 这些发现强调了在IBD患者群体中需要加强对HCoV-NL63的预防措施.
- 需要进一步的研究来阐明导致这种敏感性增加的潜在机制.
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