相关实验视频

Updated: Jun 19, 2026

A Simple Fractionated Extraction Method for the Comprehensive Analysis of Metabolites, Lipids, and Proteins from a Single Sample
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A Simple Fractionated Extraction Method for the Comprehensive Analysis of Metabolites, Lipids, and Proteins from a Single Sample

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在人体血中使用经过验证的逆相UHPSFC/MS/MS对较少极性脂质进行全面的定量分析

Zuzana Lásko1, Veronika Šubrtová1, Ondřej Peterka1

  • 1Department of Analytical Chemistry, Faculty of Chemical Technology, University of Pardubice, Studentská 573, 53210 Pardubice, Czech Republic.

Analytical chemistry
|November 24, 2025
PubMed
概括

这项研究引入了一种新方法,用于分析人体血中较少的极性脂质,使用逆相超高性能超临界流体染色学-并列质谱法 (RP-UHPSFC/MS/MS). 经过验证的技术允许高通量脂质分析和各种脂质物种的准确量化.

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High-Resolution Mass Spectrometry (HRMS)01:15

High-Resolution Mass Spectrometry (HRMS)

The resolution of a mass spectrometer depends on the efficiency of separating ions with different ion masses. The mass of an atom is approximated to the sum of the masses of protons and neutrons inside, considering the masses of protons and neutrons as equal. However, the masses of the proton (1.6726 × 10−24 g) and neutron (1.6749 × 10−24 g) are not truly equal. There is a minor error in the expression of atomic masses relative to the simplest atom of hydrogen. For example, the mass of helium...