由多菌株益生菌联盟发酵的牛奶衍生出的生物活性的抗氧化,抗糖尿病和抗菌活性
Hina Maniya1,2, Brij Pal Singh3, Vijay Kumar1,4
1Postbiotics and Foodomics Lab, School of Science, RK University, Rajkot, India.
Molecular nutrition & food research
|November 24, 2025
概括
使用本土益生菌联盟发酵牛奶显著增加生物活性 (BAP) 的健康益处. 与单个菌株相比,这些增强的BAP显示出更好的抗糖尿病,抗氧化和抗微生物特性.
科学领域:
- 食品科学与技术 食品科学与技术
- 微生物学 微生物学
- 生物化学 生物化学
背景情况:
- 牛奶衍生生物活性 (BAP) 具有显著的健康益处.
- 来自本土益生菌联盟的BAP的多生物活性潜力尚未得到充分探索.
- 由于未开发的BAP潜力,治疗应用有限.
研究的目的:
- 调查与单个菌株相比,使用本地益生菌联盟发酵牛奶是否会增加BAP活动.
- 以其α-氨酶抑制,激素清除和抗菌有效性来表征BAP.
- 探索增强BAP的潜力,以管理慢性疾病和感染.
主要方法:
- 使用Bacillus spizizenii BAB 7915和Bacillus subtilis BAB 7918联盟进行牛奶发酵.
- 在发酵后净化BAP (≤10kDa).
- 使用RP-HPLC和OHRLCMS/MS进行表征.
- 评估α-氨酶抑制,ABTS激素清除和抗菌活性 (MIC).
主要成果:
- 联盟发酵显著增强了BAP的生物活性.
- 联盟的BAP表现出优异的α-氨酶抑制 (90.80%) 和ABTS清理 (54.17%).
- 观察到对测试的病原体具有强烈的抗微生物活性 (MIC 2.5-5μg/mL).
结论:
- 土著益生菌联盟高效地生产具有增强多生物活性的牛奶BAP.
- 这些增强的BAP显示出对管理2型糖尿病,氧化应激和感染的前景.
- 需要进一步研究分离,结构功能,体内疗效和封装.
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