Jove
Visualize
联系我们

相关概念视频

Enzyme-Linked Immunosorbent Assay01:33

Enzyme-Linked Immunosorbent Assay

17.2K
In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen.  Enzyme-substrate reaction allows the antigen to be visualized or...
17.2K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Stable Heavy Body Shape Throughout the Life Course Increases the Risk of Chronic Kidney Disease.

Journal of renal nutrition : the official journal of the Council on Renal Nutrition of the National Kidney Foundation·2026
Same author

Electrochemical enhancement of sulfite-induced phosphate release from iron phosphate with concurrent micropollutant degradation in water.

Water research·2026
Same author

Microscale thermophoresis sensor for homogeneous and one-step protein detection based on dual aptamers binding induced DNA switch.

Talanta·2026
Same author

An exposome-wide association study of hyperuricemia through a rural cohort study and to predict risk factors.

International journal of biometeorology·2026
Same author

Evaluating and comparing the predictive ability with hypertension risk among obesity indicators: a prospective cohort integrating Mendelian randomization analysis.

Frontiers in nutrition·2025
Same author

Prevalence and healthy life expectancy among elderly with mild cognitive impairment in Chinese rural areas.

Journal of Alzheimer's disease : JAD·2025
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关实验视频

Updated: Jan 10, 2026

Author Spotlight: Advancements in DNA Nanosensors – Addressing Sensitivity and Selectivity Challenges in Molecular Detection
07:16

Author Spotlight: Advancements in DNA Nanosensors – Addressing Sensitivity and Selectivity Challenges in Molecular Detection

Published on: February 9, 2024

1.5K

一种基于DNA酶放大器的免疫试验,用于小分子检测.

Han Pang1,2, Qiang Zhao1,2,3

  • 1State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing, 100085, China. qiangzhao@rcees.ac.cn.

The Analyst
|November 24, 2025
PubMed
概括

这项研究引入了一种基于DNAzyme放大器的新型免疫试验,用于敏感的小分子检测. 这种方法提供了一种简单,有选择性和高度敏感的方法来检测诸如狄戈辛和叶酸等分子.

更多相关视频

Computer Numerical Control Micromilling of a Microfluidic Acrylic Device with a Staggered Restriction for Magnetic Nanoparticle-Based Immunoassays
09:58

Computer Numerical Control Micromilling of a Microfluidic Acrylic Device with a Staggered Restriction for Magnetic Nanoparticle-Based Immunoassays

Published on: June 23, 2022

2.6K
Snap Chip for Cross-reactivity-free and Spotter-free Multiplexed Sandwich Immunoassays
10:44

Snap Chip for Cross-reactivity-free and Spotter-free Multiplexed Sandwich Immunoassays

Published on: November 13, 2017

6.8K

相关实验视频

Last Updated: Jan 10, 2026

Author Spotlight: Advancements in DNA Nanosensors – Addressing Sensitivity and Selectivity Challenges in Molecular Detection
07:16

Author Spotlight: Advancements in DNA Nanosensors – Addressing Sensitivity and Selectivity Challenges in Molecular Detection

Published on: February 9, 2024

1.5K
Computer Numerical Control Micromilling of a Microfluidic Acrylic Device with a Staggered Restriction for Magnetic Nanoparticle-Based Immunoassays
09:58

Computer Numerical Control Micromilling of a Microfluidic Acrylic Device with a Staggered Restriction for Magnetic Nanoparticle-Based Immunoassays

Published on: June 23, 2022

2.6K
Snap Chip for Cross-reactivity-free and Spotter-free Multiplexed Sandwich Immunoassays
10:44

Snap Chip for Cross-reactivity-free and Spotter-free Multiplexed Sandwich Immunoassays

Published on: November 13, 2017

6.8K

科学领域:

  • 生物化学 生物化学
  • 分析化学 分析化学
  • 分子生物学分子生物学

背景情况:

  • 小分子检测对于药物发现,诊断,食品安全和环境监测至关重要.
  • 现有的小分子检测方法在选择性和灵敏性方面经常面临挑战.
  • 开发强大高效的检测策略仍然是一个重要的研究重点.

研究的目的:

  • 开发一种敏感和选择性的免疫试验,用于检测小分子.
  • 为了利用DNAzyme的催化活性在免疫试验中进行信号放大.
  • 为了证明拟议方法用于检测特定小分子的有效性.

主要方法:

  • 设计了一种基于DNAzyme放大器的免疫试验,将抗体-抗原结合与DNAzyme催化进行了整合.
  • 用抗原标记的10-23DNAzyme被用来与微板上的固定抗体结合.
  • DNA酶催化了光标记基质的裂变,产生了根据目标分子度的强化光信号.

主要成果:

  • 免疫试验成功地检测出小分子,特别是毒素和叶酸,在皮科莫尔度.
  • 该方法在识别目标小分子方面表现出高的特异性.
  • 该测试证明在复杂的样本矩阵中检测小分子是有效的.

结论:

  • 开发的基于DNAzyme放大器的免疫试验为小分子检测提供了一种简单,敏感和选择性的方法.
  • 这一策略有效地将免疫试验的特异性与DNAzymes的放大功率结合起来.
  • 这些发现激发了针对各种应用的基于DNA酶的多功能免疫试验的开发.