在肠道和肠外Bacteroides fragilis菌株中,抗菌素耐药性广泛存在
Marvic Carrillo Terrazas1, Renee E Oles1,2, Luke R Loomis1
1Department of Pathology, University of California, San Diego, La Jolla, California, USA.
肠道微生物 Bacteroides fragilis 在肠道和临床环境中表现出类似的抗菌素耐药性 (AMR) 水平. 肠道菌株可能储存耐药基因,可能使感染复杂化.
科学领域:
- 微生物学 微生物学
- 基因组学就是基因组学.
- 传染性疾病 传染性疾病
背景情况:
- 细菌菌 (Bacteroides fragilis) 是一个关键的肠道共生物,与宿主健康有关.
- 它也是临床感染的常见原因,抗菌素耐药性 (AMR) 越来越令人担忧.
- 抗药性耐药性和B. fragilis的隔离部位之间的联系尚未得到充分理解.
研究的目的:
- 为了比较肠道和肠外B. fragilis分离物之间的AMR表型和基因型.
- 为了确定临床B. fragilis菌株是否具有较高的耐药性决定因素负担.
- 调查肠道菌株作为AMR的储存库的潜力.
主要方法:
- 现型抗微生物敏感性测试 (MICs).
- 对AMR基因的基因组分析.
- 微生物全基因组关联研究 (mGWAS).
主要成果:
- 在肠道和肠外B. fragilis分离物之间观察到可比的AMR敏感性概况和基因含量.
- 在两个位点的分离物中,β-乳酸酶活性是普遍存在的 (~70%).
- mGWAS确定了已知的和新的AMR相关的遗传元素,包括结合性转位子和调节基因.
结论:
- 肠道B. fragilis菌株含有临床相关的AMR决定因素,作为潜在的储存库.
- 开始性肠道细菌中广泛存在的AMR可以使肠外感染的治疗复杂化.
- 监测肠道微生物群中的AMR至关重要.
更多相关视频
08:33Quantitative Polymerase Chain Reaction-based Analyses of Murine Intestinal Microbiota After Oral Antibiotic Treatment
Published on: November 17, 2018
12:58A Protocol to Characterize the Morphological Changes of Clostridium difficile in Response to Antibiotic Treatment
Published on: May 25, 2017
相关概念视频
Development of Antibiotic Resistance
Bacterial Phylum Bacteroidota
Gene Regulation in Microbial Communities: Quorum Sensing
Drugs Affecting GI Tract Motility: Antimicrobials as Antidiarrheal Agents
Defense Against Bacterial Pathogens
Phagocytes
Phagocytes are the frontline soldiers of the immune system. They include neutrophils and macrophages. Neutrophils are the most abundant type of white blood cell and are quickly mobilized to the site of infection. Macrophages are larger cells that patrol...
Bacterial Flora of the Large Intestine
The normal gut flora of the colon plays a critical role in generating essential vitamins such as vitamins K, B5, and B7.
