Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Transgenic Plants02:50

Transgenic Plants

8.4K
Recombinant DNA technology called transgenesis is often used to add a foreign gene or remove a detrimental gene from an organism. Such genetically modified organisms are called transgenic organisms.
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...
8.4K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Chromosome architecture affects virulence and competitiveness in <i>Agrobacterium tumefaciens</i> C58.

Science advances·2025
Same author

Enhancing <i>Agrobacterium</i>-mediated plant transformation efficiency through improved ternary vector systems and auxotrophic strains.

Frontiers in plant science·2024
Same author

Immune cell infiltration and drug response in glioblastoma multiforme: insights from oxidative stress-related genes.

Cancer cell international·2024
Same author

Periodontitis and osteoporosis: a two-sample Mendelian randomization analysis.

Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas·2024
Same author

Diagnostic Performance of Angiography-Derived Quantitative Flow Ratio in Complex Coronary Lesions.

Circulation. Cardiovascular imaging·2024
Same author

Morusin Alleviates Aortic Valve Calcification by Inhibiting Valve Interstitial Cell Senescence Through Ccnd1/Trim25/Nrf2 Axis.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2024

相关实验视频

Updated: Jan 10, 2026

Author Spotlight: Optimized Transformation Protocol for Chlorella vulgaris Using Agrobacterium tumefaciens
08:00

Author Spotlight: Optimized Transformation Protocol for Chlorella vulgaris Using Agrobacterium tumefaciens

Published on: October 27, 2023

4.7K

图片: 综合介导的 农菌 基因组工程

Ephraim Aliu1,2,3, Liang-Chun Chen1,2,3, Keunsub Lee1,2

  • 1Department of Agronomy, Iowa State University, Ames, IA, United States.

Frontiers in microbiology
|November 24, 2025
PubMed
概括

研究人员优化了CRISPRRNA引导的转化酶系统,INTEGRATE,用于精确的Agrobacterium基因组工程. 这种标准化协议有助于为植物生物技术应用开发Agrobacterium菌株.

关键词:
亚博体育ios 亚博体育ios 亚博体育ios 亚博体育ios这种细菌是Agrobacterium spp.这种细菌名为Agrobacterium tumefaciens.在CRISPR的整合过程中,克雷洛克斯 (Cre-lox) 是一个植物.解除武装 解除武装大量的DNA片段被删除.有针对性的DNA插入.

更多相关视频

Author Spotlight: Streamlining Rice Breeding with CRISPR/Cas for Obtaining Optimal Phenotypic and Agronomic Traits
09:43

Author Spotlight: Streamlining Rice Breeding with CRISPR/Cas for Obtaining Optimal Phenotypic and Agronomic Traits

Published on: January 3, 2025

3.3K
Site-specific Bacterial Chromosome Engineering: &#934;C31 Integrase Mediated Cassette Exchange (IMCE)
08:21

Site-specific Bacterial Chromosome Engineering: ΦC31 Integrase Mediated Cassette Exchange (IMCE)

Published on: March 16, 2012

16.1K

相关实验视频

Last Updated: Jan 10, 2026

Author Spotlight: Optimized Transformation Protocol for Chlorella vulgaris Using Agrobacterium tumefaciens
08:00

Author Spotlight: Optimized Transformation Protocol for Chlorella vulgaris Using Agrobacterium tumefaciens

Published on: October 27, 2023

4.7K
Author Spotlight: Streamlining Rice Breeding with CRISPR/Cas for Obtaining Optimal Phenotypic and Agronomic Traits
09:43

Author Spotlight: Streamlining Rice Breeding with CRISPR/Cas for Obtaining Optimal Phenotypic and Agronomic Traits

Published on: January 3, 2025

3.3K
Site-specific Bacterial Chromosome Engineering: &#934;C31 Integrase Mediated Cassette Exchange (IMCE)
08:21

Site-specific Bacterial Chromosome Engineering: ΦC31 Integrase Mediated Cassette Exchange (IMCE)

Published on: March 16, 2012

16.1K

科学领域:

  • 微生物学 微生物学
  • 分子生物学分子生物学
  • 生物技术是生物技术.

背景情况:

  • 对Agrobacterium菌株的精确工程对于推进植物生物技术至关重要.
  • 克里斯普尔RNA引导的转化酶系统INTEGRATE提供了一种有效的基因修饰方法,用于Agrobacterium.
  • 在Agrobacterium菌株工程中INTEGRATE的当前应用需要进一步的探索和标准化.

研究的目的:

  • 为在Agrobacterium中使用INTEGRATE系统提供标准化和优化的工作流程.
  • 解决和克服与INTEGRATE实施相关的共同挑战,包括crRNA设计,转换效率和载体驱逐.
  • 扩大Agrobacterium的基因工程工具包,以支持功能基因组学和菌株开发.

主要方法:

  • 在Agrobacterium中开发一个标准化的CRISPR-RNA引导转化酶 (INTEGRATE) 系统应用协议.
  • 优化目标部位选择,crRNA间隔器克隆和Agrobacterium转换程序.
  • 实施选方法以针对性插入,Cre/loxP介导的删除和载体去除.

主要成果:

  • 通过使用INTEGRATE系统在Agrobacterium中进行向基因组修改,建立了一个强大的工作流程.
  • 该协议成功地证明了Agrobacterium rhizogenes K599的化,通过删除T-DNA区域并创建auxotrophy.
  • 开发的方法提高了Agrobacterium基因组工程的效率和可重复性.

结论:

  • 优化的INTEGRATE协议为执行Agrobacterium基因组工程的研究人员提供了宝贵的资源.
  • 这种标准化的工作流程有助于为植物转化应用开发改进的Agrobacterium菌株.
  • 这项研究促进了在Agrobacterium中更广泛地采用先进的基因工程工具,推动了植物生物技术的创新.