在不同的碳来源下,对Akkermansia muciniphila和Bifidobacterium物种共同培养的蛋白质学研究
Jordy Evan Sulaiman1,2, Yuewei Zhan3, Shuchen Wang3
1Department of Health Technology and Informatics, The Hong Kong Polytechnic University, Kowloon, Hong Kong SAR, China.
Frontiers in microbiology
|November 24, 2025
概括
食纤维,如阿拉比诺西兰,可以提高益生菌Akkermansia muciniphila和Bifidobacterium物种的疗效. 这项研究表明,特定的营养素如何塑造益生菌的生长和蛋白质表达,改善肠道屏障功能.
科学领域:
- 微生物学 微生物学
- 肠道微生物组研究研究
- 代谢健康 代谢健康
背景情况:
- Akkermansia muciniphila和Bifidobacterium物种是宿主代谢和治疗代谢疾病的关键益生菌.
- 将这些益生菌一起配制可以提高疗效,但它们在不同营养条件下的相互作用尚未得到充分理解.
研究的目的:
- 研究不同碳来源对A. muciniphila在与B. longum和B. breve共同培养时对其生长和蛋白质表达的影响.
- 探索食纤维如何影响这些益生菌物种的相互作用和功能.
主要方法:
- 使用无标签的定量蛋白质组学分析了A. muciniphila,B. longum和B. breve在与各种碳来源 (单糖,木) 的共同培养中.
- 评估了生长特征和蛋白质表达变化.
- 研究人员选了健康相关的食纤维,包括阿拉比诺西兰,以确定它们对益生菌生长和相互作用的影响.
主要成果:
- 根据碳来源,A. muciniphila与B. breve和B. longum表现出不同的生长模式.
- 当与A. muciniphila共同培养时,B. longum的丰富性下降,而B. breve则减少了A. muciniphila在单糖介质中的丰富性.
- 阿拉比诺克西兰有选择地促进了B. longum的生长,在共同培养中,增强了A. muciniphila的生长和Amuc_1100蛋白质的表达,改善了肠道上皮屏障的完整性.
结论:
- 甘氨酸显著影响共同培养的A. muciniphila和Bifidobacterium物种的生长和蛋白质组概况.
- 食纤维,如阿拉比诺西兰,可以被利用来优化益生菌群落的功能协同作用,以提高健康益处.
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