在美国德克萨斯州正在发生的麻疹疫情期间,在不同地点区分空间信息化废水
Laura M Langan1, Fallon L Bain2,3, Christine C Snow2,3
1Department of Environmental Health Sciences, Arnold School of Public Health, University of South Carolina, 921 Assembly St., Columbia, South Carolina 28208, United States.
ACS environmental Au
|November 24, 2025
概括
使用RT-qPCR和RT-dPCR检测到麻疹RNA的废水监测,显示RT-qPCR.
科学领域:
- 环境微生物学环境微生物学
- 分子诊断学 分子诊断学
- 公共卫生监督是对公共卫生的监督.
背景情况:
- 基于废水的流行病学和监测 (WBE/WBS) 对于监测传染病至关重要.
- 反转录定量聚合酶连锁反应 (RT-qPCR) 和RT数字PCR (RT-dPCR) 是常见的WBE/WBS平台,可访问性和经济性不同.
- RT-qPCR更为广泛和经济,使其成为监测各种病原体的常见选择,包括SARS-CoV-2.
研究的目的:
- 为了比较RT-qPCR和RT-dPCR在废水中检测麻疹病毒RNA的性能.
- 评估这些方法在活跃疫情期间区分麻疹菌株的有效性.
- 评估WBE/WBS对空间知情公共卫生响应的有用性.
主要方法:
- 在废水样本中检测麻疹RNA的RT-qPCR和RT-dPCR平台的比较分析.
- 使用合成DNA的两种方法的检测极限 (LOD) 的评估.
- 来自美国德克萨斯州多个废水地点的疫苗 (艾德蒙斯顿基因型A) 和野生型 (D8基因型) 麻疹RNA的分析.
主要成果:
- 无论是RT-qPCR还是RT-dPCR平台,对于麻疹RNA的检测极限 (LODs) 都具有可比性.
- 与RT-dPCR相比,RT-qPCR显示疫苗菌株麻疹RNA的检测率更高.
- 在平台之间观察到相当于野生型麻疹RNA的检测,量化基因拷贝的变化.
- 在RT-qPCR和RT-dPCR平台之间,在基因拷贝量化方面发现了显著的差异.
结论:
- 基于废水的监测对于在疫情爆发期间空间区分麻疹是有效的,无论使用PCR平台.
- 通过RT-qPCR检测,在废水中的疫苗菌株麻疹RNA的检测率更高.
- 平台之间的量化差异凸显了WBE/WBS的方法选择的重要性.
- 在麻疹爆发期间,WBE/WBS为快速反应和公共卫生决策提供了有价值的数据.
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