Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

1.6K
The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
1.6K
CRISPR and crRNAs02:53

CRISPR and crRNAs

18.7K
Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
18.7K
CRISPR01:59

CRISPR

57.4K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
57.4K
Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

6.6K
Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
6.6K
Homologous Recombination02:31

Homologous Recombination

62.5K
The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
62.5K
The Antiviral System of Bacteria and Archaea: CRISPR01:23

The Antiviral System of Bacteria and Archaea: CRISPR

601
CRISPR stands for Clustered Regularly Interspaced Short Palindromic Repeats is a adaptive immune system found in bacteria and archaea that protects against viral infections. This system enables prokaryotic cells to identify, remember, and neutralize foreign genetic elements, primarily bacteriophages, by storing fragments of the invader’s DNA as a genetic memory.The CRISPR immune response begins during an initial infection. Cas (CRISPR-associated) proteins play a central role in this...
601

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Systems engineering of engineered live biotherapeutics: A discovery-to-translation framework for streamlining microbiome therapeutic development.

Journal of controlled release : official journal of the Controlled Release Society·2026
Same author

Lumpectomy Margins and Local Recurrence in DCIS: Results From the NRG Oncology/NSABP B-35 Randomized Clinical Trial.

JAMA surgery·2026
Same author

Nanoengineered All-Cellulose Bilayer Barrier Papers for High-Performance and Recyclable Food Packaging.

Chem & bio engineering·2026
Same author

Carbon monoxide-releasing molecule-3 eradicates mature <i>Enterococcus faecalis</i> biofilms and inhibits recolonization.

Journal of biomaterials applications·2026
Same author

VMP1 forms a Ca<sup>2+</sup> release channel essential for postnatal heartbeat.

Science advances·2026
Same author

Biomolecular "lock-key" model-inspired cage/benzene ring structure chain-extension matching strategy for high performance of linear waterborne polyurethane toward application in recyclable soft robotics.

Journal of colloid and interface science·2026

相关实验视频

Updated: Jan 10, 2026

QTL Mapping and CRISPR/Cas9 Editing to Identify a Drug Resistance Gene in Toxoplasma gondii
11:37

QTL Mapping and CRISPR/Cas9 Editing to Identify a Drug Resistance Gene in Toxoplasma gondii

Published on: June 22, 2017

16.7K

可编程的结合式CRISPR干扰向肠道中的基因毒素.

Brian Hamp1, Hania Timek1, Chengyuan Fang1,2

  • 1Department of Biomedical Engineering, College of Engineering and School of Medicine, University of Michigan, Ann Arbor, MI, 48109, United States.

bioRxiv : the preprint server for biology
|November 24, 2025
PubMed
概括

我们开发了一种由等离子体传递的CRISPR干扰 (CRISPRi) 系统,以沉默大肠杆菌中的胆固醇蛋白生产,降低小鼠结直肠癌风险.

更多相关视频

Application of CRISPR Interference CRISPRi for Gene Silencing in Pathogenic Species of Leptospira
14:49

Application of CRISPR Interference CRISPRi for Gene Silencing in Pathogenic Species of Leptospira

Published on: August 14, 2021

5.6K
Gene Digital Circuits Based on CRISPR-Cas Systems and Anti-CRISPR Proteins
10:46

Gene Digital Circuits Based on CRISPR-Cas Systems and Anti-CRISPR Proteins

Published on: October 18, 2022

2.2K

相关实验视频

Last Updated: Jan 10, 2026

QTL Mapping and CRISPR/Cas9 Editing to Identify a Drug Resistance Gene in Toxoplasma gondii
11:37

QTL Mapping and CRISPR/Cas9 Editing to Identify a Drug Resistance Gene in Toxoplasma gondii

Published on: June 22, 2017

16.7K
Application of CRISPR Interference CRISPRi for Gene Silencing in Pathogenic Species of Leptospira
14:49

Application of CRISPR Interference CRISPRi for Gene Silencing in Pathogenic Species of Leptospira

Published on: August 14, 2021

5.6K
Gene Digital Circuits Based on CRISPR-Cas Systems and Anti-CRISPR Proteins
10:46

Gene Digital Circuits Based on CRISPR-Cas Systems and Anti-CRISPR Proteins

Published on: October 18, 2022

2.2K

科学领域:

  • 微生物学 微生物学
  • 遗传学 是一个遗传学.
  • 在瘤学瘤学.

背景情况:

  • 科利巴克丁是大肠杆菌*的代谢物,与早期结直肠癌有关.
  • 目前没有一种疗法可以直接抑制胆固醇菌素的产生.

研究的目的:

  • 设计一种用于向菌素生物合成基因沉默的新型系统.
  • 评估该系统在降低*pks*+*E. coli*基因毒性和相关瘤发生方面的有效性.

主要方法:

  • 设计了一种自我传播的结合性等离子体,以提供CRISPR干扰 (CRISPRi).
  • 使用CRISPRi使大肠杆菌*菌株中的*pks*+*E. coli*生物合成基因沉默.
  • 在小鼠模型中评估了基因毒性,殖民化,DNA损伤和瘤发生.

主要成果:

  • 克里斯皮尔系统有效地抑制了 colibactin 的产生,并消除了 *pks*+ *E. coli* 的基因毒性,而不诱导耐药性突变.
  • 在小鼠中,结合介导的CRISPRi减少了DNA损伤,大肠杆菌殖民和瘤发生.
  • 该平台被扩展到使第二个致病代谢物沉默,证明了广泛的适用性.

结论:

  • 结合介导的CRISPRi是一种有前途的策略,可以中和胆固醇菌素和其他微生物代谢产物.
  • 这种方法提供了一个可编程的实时生物治疗策略,用于肠道健康和疾病预防.
  • 开发的系统在临床前模型中表现优于现有的药物抑制剂.