年龄和性别驱动的转录和新陈代谢多样性在小鼠败血症后的髓衍生抑制细胞中
Christine Rodhouse1,2, Dayuan Wang1,3, Hongru Tang1,3
1Sepsis and Critical Illness Research Center, University of Florida College of Medicine, Gainesville, FL, USA.
bioRxiv : the preprint server for biology
|November 24, 2025
概括
败血症导致免疫抑制,由髓质衍生抑制细胞 (MDSCs) 驱动. 这项研究揭示了年龄和性别显著改变MDSC反应,突出了个性化败血症治疗的潜力.
科学领域:
- 免疫学 免疫学 免疫学
- 基因组学就是基因组学.
- 代谢学 代谢学 代谢学
背景情况:
- 败血症导致免疫失调和慢性严重疾病.
- 骨髓系衍生抑制细胞 (MDSCs) 介导败血症诱导的免疫抑制.
- 年龄和性别对败血症中MDSC功能的影响尚不清楚.
研究的目的:
- 在败血症期间,研究髓质衍生抑制细胞 (MDSC) 现型的年龄和性别特异性差异.
- 定义MDSCs的转录和代谢状态在年轻与年老的雄性和雌性小鼠中.
主要方法:
- 从年轻和老年雄性和雌性小鼠的脏白细胞单细胞RNA测序,这些小鼠接受了小鼠败血症模型.
- 对MDSC扩张,转录组,正规通路,RNA速度和线粒体代谢的分析.
- 药物2细胞分析以确定队列特定的药物标.
主要成果:
- 在MDSC扩展和转录资料中观察到显著的年龄和性别差异.
- 根据年龄和性别,在规范路径激活,RNA速度和线粒体代谢中发现了明显的变化.
- 败血症诱导的MDSC生物学受到宿主年龄和性别的深刻影响,不同的队列出现了独特的药物标配置文件.
结论:
- 年龄和性别是骨髓原抑制细胞 (MDSC) 在败血症中的行为和功能的关键决定因素.
- 了解这些特定的MDSC表型对于开发有效的免疫调节疗法至关重要.
- 针对MDSC的个性化治疗策略有望改善败血症患者的治疗结果.
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