在青春期后的小鼠CA1反向方向中,LTP与激发抑制平衡的平行昼夜类似振荡
bioRxiv : the preprint server for biology
|November 24, 2025
概括
突触可塑性,称为长期强化 (LTP),表现出与激发-抑制 (E/I) 比率相关的每日节律,而不是光明-黑暗周期. 这种E/I比率会影响海马体中的LTP大小.
科学领域:
- 神经科学是一个神经科学.
- 时间生物学 时间生物学
- 突触性可塑性 突触性可塑性
背景情况:
- 长期增强 (LTP) 是学习和记忆的关键机制.
- 昼夜节律显著影响夜行动物的海马突触可塑性.
- 之前的研究表明,在夜生活物种的黑暗阶段,LTP更强大.
研究的目的:
- 研究调节海马体LTP昼夜控制的机制.
- 为了确定LTP的容量是否在明暗周期之间有所不同.
- 检查激发-抑制 (E/I) 比率在每日LTP波动中的作用.
主要方法:
- 在小鼠海马CA1区域的电生理学记录.
- 使用TBS-LTP和配对协议诱导LTP.
- 操纵抑制性神经传递.
- 在野生型 (WT) 和APP/PS1老鼠阿尔茨海默病 (AD) 模型中的评估.
主要成果:
- 在明暗周期之间,表达LTP的能力并不不同.
- TBS-LTP的大小与E/I比率的每日波动相关,在黑暗阶段更高.
- 抑制的阻断消除了TBS-LTP的明暗差异,而伴侣诱导的LTP显示没有周期差异.
- 在APP/PS1 AD模型中,无论是E/I比率还是TBS-LTP都没有每日变化.
- 在WT小鼠中,E/I比率和TBS-LTP的每日振荡在青春期后逆转了方向.
结论:
- 海马LTP大小的循环调节是由每日E/I比率的变化介导的,而不是改变的可塑性容量.
- 在AD小鼠模型中,E/I比率和相关的LTP模式被破坏.
- 发生在青春期后的海马体可塑性的昼夜控制中的发育开关.
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