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Updated: Jan 10, 2026

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混合固体-液体光学可实现可扩展,高分辨率,多浸泡光片显微镜

Cheng Gong^1,2, Pauline Affatato¹, Matt Fay³

¹Departments of Biological Sciences, Columbia University, New York, NY, USA.

bioRxiv : the preprint server for biology

|November 24, 2025

概括

研究人员开发了一种新的成像框架 (HySIL) 和设备 (SCOPE/Super-SCOPE),以实现大型生物样本的高分辨率3D成像. 这在现代生物学中推进了可扩展,数据驱动的发现.

科学领域:

生物物理学的生物物理.
光学工程是指光学工程.
细胞生物学细胞生物学

背景情况:

高内容的3D成像对于数据驱动的生物发现至关重要.
目前使用沉浸或空气目标的方法在大样本的可扩展性,成本和分辨率方面存在局限性.

研究的目的:

开发一种可扩展和负担得起的高分辨率3D成像解决方案,用于分子到器官尺度.
克服传统显微镜在成像完整,清除或扩展生物标本方面的局限性.

主要方法:

介绍了混合固体-液体浸泡镜头 (HySIL) 框架,将固体光学与指数匹配的液体相结合.
基于HySIL的SCOPE和Super-SCOPE客观不可知成像设备的开发.
与低成本光片显微镜 (LSM) 平台的集成.

主要成果:

通过使用廉价的空中镜头,实现了微米次的横向分辨率 (<0.75微米),使用长距离的工作距离 (>30毫米).
证明了各种生物样本的可扩展,亚细胞分辨率的体积映射,包括大脑,有机体和组织病理学.
启用了多沉浸和多色成像功能.

结论:

HySIL和SCOPE为可扩展的高分辨率体积成像提供了一个可访问的基础.
开发的系统通过能够详细地绘制大型生物结构来显著推进数据驱动的生物发现.
为研究和诊断中的高内容3D成像提供了具有成本效益的替代方案.

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