一个可扩展的蛋白质基因组框架,用于剖析原发性免疫细胞中的光信号通路
Christian M Beusch1,2,3,4,5, Carolyn Morningstar1,2,3,4,6, Marc Semaan7,8
1Pathology Advanced Translational Research Unit, Department of Pathology and Laboratory Medicine, Emory University School of Medicine, Atlanta, GA, USA.
bioRxiv : the preprint server for biology
|November 24, 2025
概括
我们开发了一个蛋白质基因组框架和简单的Phos工作流程来绘制细胞信号通路. 这种方法系统地分析了人体免疫细胞中细胞因子诱导的信号,推进了转化免疫学.
科学领域:
- 免疫学 免疫学 免疫学
- 蛋白质组学是指蛋白质组学.
- 系统生物学 系统生物学
背景情况:
- 细胞反应依赖于复杂的信号网络,通过后翻译性修饰来调制.
- 现有的研究这些途径的方法缺乏系统的因果架构映射的规模和吞吐量.
- 了解细胞因子诱导的信号传递对于免疫学和治疗发展至关重要.
研究的目的:
- 为系统地绘制细胞因子诱导的信号通路提供一个综合蛋白质基因组框架.
- 介绍simplePhos,一个简化的蛋白学工作流程,用于可扩展的,时间解决的分析.
- 展示一种强大的策略,用于询问人类免疫细胞中的光信号网络.
主要方法:
- 开发了一个集成的蛋白质基因组框架,结合了遗传干扰和高通量蛋白质组学.
- 利用simplePhos,一个低输入的蛋白学工作流程,用于可扩展的分析.
- 在各种炎症刺激下,将框架和工作流应用于原发性人类T细胞和髓状细胞.
主要成果:
- 建立了主要免疫细胞中细胞因子诱导信号的基础蛋白质基因组数据集.
- 在多种炎症刺激 (干扰子,LPS,仙台病毒) 中验证了simplePhos管道.
- 通过使用集成框架,成功地绘制了I型干扰子信号的关键信号节点.
结论:
- 集成的蛋白质基因组框架和简单的Phos工作流可以系统地映射原发性免疫细胞中的信号通路.
- 这种方法为光信号网络的机械质询提供了一个强大的策略.
- 这些发现在翻译免疫学和新疗法开发方面具有广泛的应用.
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