多浸泡斜平面显微镜 (miOPM):用于高分辨率光板光显微镜的可重新配置平台.
Bingying Chen1,2, Alfred Millett-Sikking3, Seweryn Gałecki1,2,4
1Lyda Hill Department of Bioinformatics, UT Southwestern Medical Center, Dallas, TX, 75390, U.S.A.
bioRxiv : the preprint server for biology
|November 24, 2025
概括
我们开发了多浸泡斜平面显微镜 (miOPM),这是一个可适应的光板光显微镜 (LSFM) 平台. 这种多功能工具可以在各种生物样本和尺度上增强高分辨率的3D成像.
科学领域:
- 显微镜的使用方法
- 生物物理学的生物物理.
- 光学工程是指光学工程.
背景情况:
- 光板光显微镜 (LSFM) 提供了温和,快速和高效的体积成像.
- 简单的SFM变体通常是狭窄的优化,限制了广泛的适用性.
- 对于各种生物样本,存在适应性的LSFM平台的需求.
研究的目的:
- 介绍多浸泡斜平面显微镜 (miOPM) 作为一个可适应的LSFM平台.
- 展示miOPM在各种空间尺度上进行高分辨率成像的能力.
- 扩大LSFM的适应性和易用性,用于高级3D成像.
主要方法:
- 开发了多浸泡斜平面显微镜 (miOPM).
- 实现了油,水和空气目标的无互换性.
- 在1.33-1.51的折射率范围内验证了性能.
主要成果:
- miOPM支持从亚细胞动态到整个生物体和清除的组织的高分辨率成像.
- 在广泛的折射率范围内实现了衍射有限的性能.
- 与标准样品安装,清除协议和高通量3D成像的证明兼容性.
结论:
- 该miOPM显著扩大了LSFM的适应性和易用性.
- miOPM可在多个尺度上对各种生物标本进行高分辨率的3D成像.
- 这种可适应的LSFM平台准备民主化先进的三维成像.
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