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Updated: Jan 10, 2026

CRISPR Epigenome Editing in Human Cells using Plasmid DNA Transfection and mRNA Nucleofection Delivery
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CRISPR Epigenome Editing in Human Cells using Plasmid DNA Transfection and mRNA Nucleofection Delivery

Published on: May 30, 2025

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核定位信号使细胞能够输送抗CRISPR蛋白来控制基因组编辑

Axel O Vera, Franklin J Avilés-Vázquez, Taekjip Ha

    bioRxiv : the preprint server for biology
    |November 24, 2025
    PubMed
    概括

    我们开发了6×NLS-Acr,一种细胞透的抗CRISPR蛋白 (Acr),以提高CRISPR-Cas基因组编辑精度. 这种抑制剂迅速进入细胞,显著提高编辑特异性和治疗潜力.

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    A Standard Methodology to Examine On-site Mutagenicity As a Function of Point Mutation Repair Catalyzed by CRISPR/Cas9 and SsODN in Human Cells10:07

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