Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Atomic Force Microscopy01:08

Atomic Force Microscopy

4.3K
Atomic force microscopy (AFM) is a type of scanning probe microscopy that can analyze topographic details of various specimens like ceramics, glass, polymers, and biological samples. AFM offers over 1000 times more resolution than the optical imaging system. Images generated from AFM are three-dimensional surface profiles, offering an advantage over the flat, two-dimensional images from other imaging techniques.
The AFM Probe
The probe is regarded as the heart of any AFM setup and comprises the...
4.3K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

EasyVoid: A Handheld Coandă Effect Uroflowmeter for Accurate and Portable Measurement of Urinary Flow Dynamics.

IEEE journal of translational engineering in health and medicine·2026
Same author

Survival Benefits Outweigh Germline Competition Costs in Kin Chimeras.

bioRxiv : the preprint server for biology·2026
Same author

Cilia-driven epithelial folding and unfolding in an early diverging animal.

Proceedings of the National Academy of Sciences of the United States of America·2025
Same author

Fluid-Squid: DIY Multiplexed Imaging of Cells and Tissues.

bioRxiv : the preprint server for biology·2025
Same author

Ice gliding diatoms establish record-low temperature limits for motility in a eukaryotic cell.

Proceedings of the National Academy of Sciences of the United States of America·2025
Same author

Escape motility of multicellular magnetotactic prokaryotes.

Journal of the Royal Society, Interface·2024
Same journal

Layered social competition coordinates reproductive hierarchy formation in ants.

bioRxiv : the preprint server for biology·2026
Same journal

Combination epigenetic-targeted therapy increases the immunogenicity of poorly immunogenic sarcomas.

bioRxiv : the preprint server for biology·2026
Same journal

Loss of LanC-like proteins delays post-injury regeneration of aging skeletal muscles.

bioRxiv : the preprint server for biology·2026
Same journal

Integrative Transfer Network: Deep Transfer Learning Across Populations and Prediction Targets.

bioRxiv : the preprint server for biology·2026
Same journal

Confidence-supported label-free metabolic imaging with FPhaS phase autofluorescence microscopy.

bioRxiv : the preprint server for biology·2026
Same journal

Sequence-encoded autoinhibition couples mRNA decapping activity to phase separation.

bioRxiv : the preprint server for biology·2026
查看所有相关文章

相关实验视频

Updated: Jan 10, 2026

Photoactivated Localization Microscopy with Bimolecular Fluorescence Complementation BiFC-PALM
12:42

Photoactivated Localization Microscopy with Bimolecular Fluorescence Complementation BiFC-PALM

Published on: December 22, 2015

10.3K

弹性显微镜 (BaM) 是一种

A S Jijumon1, Ray Chang1,2, Manu Prakash1,3,4,5

  • 1Department of Bioengineering, Stanford University, Stanford, California, United States of America.

bioRxiv : the preprint server for biology
|November 24, 2025
PubMed
概括
此摘要是机器生成的。

弹性显微镜 (BaM) 通过将纳米颗粒轰炸到活细胞的图像,从而创建细胞内容的物理快照. 这种新的技术使空间绘制和发现细胞内未知的生物分子成为可能.

更多相关视频

A Cell-to-cell Macromolecular Transport Assay in Planta Utilizing Biolistic Bombardment
07:14

A Cell-to-cell Macromolecular Transport Assay in Planta Utilizing Biolistic Bombardment

Published on: August 27, 2010

12.3K
An Experimental Protocol for Femtosecond NIR/UV - XUV Pump-Probe Experiments with Free-Electron Lasers
09:49

An Experimental Protocol for Femtosecond NIR/UV - XUV Pump-Probe Experiments with Free-Electron Lasers

Published on: October 23, 2018

16.4K

相关实验视频

Last Updated: Jan 10, 2026

Photoactivated Localization Microscopy with Bimolecular Fluorescence Complementation BiFC-PALM
12:42

Photoactivated Localization Microscopy with Bimolecular Fluorescence Complementation BiFC-PALM

Published on: December 22, 2015

10.3K
A Cell-to-cell Macromolecular Transport Assay in Planta Utilizing Biolistic Bombardment
07:14

A Cell-to-cell Macromolecular Transport Assay in Planta Utilizing Biolistic Bombardment

Published on: August 27, 2010

12.3K
An Experimental Protocol for Femtosecond NIR/UV - XUV Pump-Probe Experiments with Free-Electron Lasers
09:49

An Experimental Protocol for Femtosecond NIR/UV - XUV Pump-Probe Experiments with Free-Electron Lasers

Published on: October 23, 2018

16.4K

科学领域:

  • 细胞生物学 细胞生物学
  • 生物物理学的生物物理.
  • 显微镜的使用方法

背景情况:

  • 光和电子显微镜提供高分辨率,但与未知的生物分子的新发现和空间绘图作斗争.
  • 像质谱学这样的无标签方法缺乏活细胞和亚细胞环境.

研究的目的:

  • 介绍弹性显微镜 (BaM) 作为一种新的成像技术.
  • 实现空间分辨率的实体采样,用于跨生物尺度的分子发现.

主要方法:

  • 用高速纳米颗粒轰炸活细胞,以捕获在水凝基板上的细胞质含量.
  • 使用SPLAT-MAP技术来保存捕获的生物分子的空间信息.
  • 使用模块化读取平台进行采集后分析,使用TEM,冷-EM,质谱和其他方法.

主要成果:

  • 在HEK细胞中发现了以前未知的CLIP170和Tau3R凝结物的组成.
  • 确定了胺-18作为这些凝结物的结构元素.
  • 证明了BaM对空间分辨率实时采样的能力.

结论:

  • 弹性显微镜 (BaM) 为活细胞建立了"物理成像"的新范式.
  • BaM为各种捕获后分析技术提供了一个模块化平台.
  • 这种方法有助于在细胞,组织和生物体中发现和空间绘制生物分子.