在WHI5促销器中的Fkh1/2结合站点数组驱动子缩放转录
Jacob Kim1,2, Shicong Xie1, Lucas Fuentes Valenzuela1
1Department of Biology, Stanford University, Stanford CA, USA.
bioRxiv : the preprint server for biology
|November 24, 2025
概括
发芽的酵母细胞通过调节Whi5蛋白水平来维持大小. 转录因子Fkh1和Fkh2结合WHI5促进体DNA,确保一致的Whi5表达,控制细胞循环的进入.
科学领域:
- 细胞生物学 细胞生物学
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
背景情况:
- 细胞大小通过与细胞分裂周期的合生长来严格调节.
- 在芽的酵母中,G1/S抑制剂Whi5蛋白被细胞生长稀释,控制细胞循环的进展.
- 细胞诞生时的一致的Whi5蛋白水平对于大小调节至关重要,通过大小独立的mRNA表达和平等的蛋白分区来实现.
研究的目的:
- 阐明 WHI5 基因尺寸独立 (子缩放) 表达的基础分子机制.
- 为了确定特定的DNA序列和转录因子,负责细胞周期期间调节WHI5转录.
主要方法:
- 在WHI5促进体区域的系统性突变发生.
- 删除FKH1和FKH2基因的分析.
- 对转录因子二分化的研究.
- 转录因子-DNA相互作用的结构预测和数学建模.
主要成果:
- WHI5的核心促进器区域 (-126至-75bp) 对于亚缩放表达至关重要.
- 这个区域包含了转录因子Fkh1和Fkh2的多个结合点.
- 这些结合位点的突变,FKH1/FKH2的删除或Fkh1/Fkh2二分化中断会损害WHI5亚缩放转录.
结论:
- WHI5的亚缩放转录由Fkh1和Fkh2的异聚合物调节.
- 这种Fkh1/2异质聚合物与WHI5核心促进体内的一系列位点合作结合.
- 这种机制确保了一致的Whi5水平,有助于精确控制发芽酵母细胞大小.
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