一个基于内部的定时器用于昼夜节律
Ye Yuan1, Amanda Linskens1, Rafael De Gouvea2
1Cellular and Molecular Biology Program, University of Michigan, Ann Arbor, MI, 48109, USA.
bioRxiv : the preprint server for biology
|November 24, 2025
概括
在Drosophila无时代 (tim) 基因中的一种新型RNA定时器,内P,通过调节mRNA核保留来控制昼夜节律. 这个内部子.
科学领域:
- 时间生物学 时间生物学
- 分子生物学分子生物学
- 在RNA生物学,RNA生物学.
背景情况:
- 循环时钟通过转录-翻译反循环控制了真核生物中的24小时节奏.
- 确切的分子机制设置昼夜周期仍然不完全理解.
研究的目的:
- 为了确定调节昼夜周期长度的新型分子计时器.
- 研究RNA处理在昼夜基因表达中的作用.
主要方法:
- 单个分子成像成像技术
- 新生RNA测序新生RNA测序
- 在CRISPR中介的基因编辑中.
- 异质的记者测定在Drosophila和人类细胞中进行.
主要成果:
- 在Drosophila无时代 (tim) 基因中的单个内子 (intron P) 作为基于RNA的分子定时器.
- 由于效率低下的拼接,Intron P会导致tim mRNAs的50%的核保留,控制周期长度.
- 移除P 内子将昼夜周期缩短至22小时;将其插入其他基因将核保留.
- RNA结合蛋白 (Hrb27C,鱼,Qkr58E-2) 调节了内子P拼接动力学.
结论:
- 时间内钟P是第一个在昼夜时钟中发现的基于内钟的分子计时器.
- 剪接动力学代表了时间基因表达中的关键调节层.
- 这种机制对发育和免疫有潜在的影响.
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