Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

RACE - Rapid Amplification of cDNA Ends02:35

RACE - Rapid Amplification of cDNA Ends

7.1K
Rapid Amplification of cDNA Ends, or RACE, is one of the most effective methods to obtain a full-length cDNA from an mRNA sequence between a known internal region to the unknown sequence at the 5’ or 3’ end. The unknown region is cloned in the cDNA by a gene-specific primer that binds the known end, and a hybrid primer that attaches a predefined anchor sequence to the unknown end of the cDNA. The sequence in between is amplified by PCR with an anchor primer and a gene-specific...
7.1K
Pre-mRNA Processing: Modification of pre-mRNA Ends01:35

Pre-mRNA Processing: Modification of pre-mRNA Ends

13.5K
In eukaryotic cells, transcripts made by RNA polymerase are modified and processed before exiting the nucleus. Unprocessed RNA is called precursor mRNA or pre-mRNA to distinguish it from mature mRNA.
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a cap to the 5' end of the growing transcript. In this process, a 5' phosphate is replaced by modified guanosine that has a methyl group attached (7-methyl guanosine). This 5' cap helps...
13.5K
Genome Annotation and Assembly03:36

Genome Annotation and Assembly

20.5K
The genome refers to all of the genetic material in an organism. It can range from a few million base pairs in microbial cells to several billion base pairs in many eukaryotic organisms. Genome assembly refers to the process of taking the DNA sequencing data and putting it all back together in a correct order to create a close representation of the original genome. This is followed by the identification of functional elements on the newly assembled genome, a process called genome annotation.
20.5K
RNA Splicing01:32

RNA Splicing

60.3K
Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
60.3K
Protein Complex Assembly02:41

Protein Complex Assembly

2.5K
2.5K
Transcription01:17

Transcription

32.4K
Transcription is the synthesis of RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in correctly synthesizing messenger RNA (mRNA). Transcriptional regulation is responsible for the differentiation of different types of cells and often for the proper cellular response to environmental signals.
Transcription Can Produce Different Kinds of RNA Molecules
In eukaryotes,...
32.4K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Augmenting transcriptome annotations through the lens of splicing evolution.

Genome research·2026
Same author

Hash functions in nucleotide sequence analysis.

Genome research·2026
Same author

Minimum flow decomposition guided by saturating subflows.

bioRxiv : the preprint server for biology·2025
Same author

MELO-ED: learning locality-sensitive multi-embeddings for edit distance.

bioRxiv : the preprint server for biology·2025
Same author

Amaranth: Enhanced Single-Cell Transcript Assembly via Discriminative Modeling of UMI Reads and Internal Reads.

bioRxiv : the preprint server for biology·2025
Same author

Accurate Reconstruction of Circular RNAs from Complex Rolling Circular Long Reads with CircPlex.

bioRxiv : the preprint server for biology·2025
Same journal

Layered social competition coordinates reproductive hierarchy formation in ants.

bioRxiv : the preprint server for biology·2026
Same journal

Combination epigenetic-targeted therapy increases the immunogenicity of poorly immunogenic sarcomas.

bioRxiv : the preprint server for biology·2026
Same journal

Loss of LanC-like proteins delays post-injury regeneration of aging skeletal muscles.

bioRxiv : the preprint server for biology·2026
Same journal

Integrative Transfer Network: Deep Transfer Learning Across Populations and Prediction Targets.

bioRxiv : the preprint server for biology·2026
Same journal

Confidence-supported label-free metabolic imaging with FPhaS phase autofluorescence microscopy.

bioRxiv : the preprint server for biology·2026
Same journal

Sequence-encoded autoinhibition couples mRNA decapping activity to phase separation.

bioRxiv : the preprint server for biology·2026
查看所有相关文章

相关实验视频

Updated: Jan 10, 2026

3' End Sequencing Library Preparation with A-seq2
12:01

3' End Sequencing Library Preparation with A-seq2

Published on: October 10, 2017

11.0K

通过划分转录开始和结束站点来增强转录组装.

Irtesam Mahmud Khan1, Xiaofei Carl Zang2,3, Ange Teng4

  • 1Department of Computer Science and Engineering, The Pennsylvania State University, 201 Old Main,University Park, 16802, PA, USA.

bioRxiv : the preprint server for biology
|November 24, 2025
PubMed
概括
此摘要是机器生成的。

新的机器学习框架Telos精确地识别了转录的开始和结束地点,以改进RNA测序组装. 这个工具可以提高各种测序技术的转录精度.

更多相关视频

A Computational Pipeline for Intergenic/Intragenic Enhancer RNA Quantification in Mouse Embryonic Stem Cells
06:02

A Computational Pipeline for Intergenic/Intragenic Enhancer RNA Quantification in Mouse Embryonic Stem Cells

Published on: October 28, 2025

414
De novo Identification of Actively Translated Open Reading Frames with Ribosome Profiling Data
08:23

De novo Identification of Actively Translated Open Reading Frames with Ribosome Profiling Data

Published on: February 18, 2022

4.1K

相关实验视频

Last Updated: Jan 10, 2026

3' End Sequencing Library Preparation with A-seq2
12:01

3' End Sequencing Library Preparation with A-seq2

Published on: October 10, 2017

11.0K
A Computational Pipeline for Intergenic/Intragenic Enhancer RNA Quantification in Mouse Embryonic Stem Cells
06:02

A Computational Pipeline for Intergenic/Intragenic Enhancer RNA Quantification in Mouse Embryonic Stem Cells

Published on: October 28, 2025

414
De novo Identification of Actively Translated Open Reading Frames with Ribosome Profiling Data
08:23

De novo Identification of Actively Translated Open Reading Frames with Ribosome Profiling Data

Published on: February 18, 2022

4.1K

科学领域:

  • 生物信息学是一种生物信息学.
  • 计算生物学 计算生物学
  • 基因组学就是基因组学.

背景情况:

  • 准确的转录组装对于理解基因表达至关重要.
  • 由于杂的RNA-seq数据,现有的方法在精确的转录开始地点 (TSS) 和转录结束地点 (TES) 检测方面扎.
  • 这种限制会影响整体的转录组装准确性.

研究的目的:

  • 开发一个新的机器学习框架,Telos,用于精确的TSS和TES检测.
  • 通过明确建模TSS和TES来提高转录组装的准确性.
  • 提供一个可以与现有的汇编器集成的转录排名工具.

主要方法:

  • 泰洛斯采用了一种两阶段的机器学习方法.
  • 第一阶段使用工程特征评分潜在的TSS和TES.
  • 第二阶段整合了站点级分数,转录丰富度和转录评分的外子统计数据.

主要成果:

  • 泰洛斯在ONT,PacBio和Illumina数据集中表现出与基线方法相比的一致的性能改进.
  • 该框架准确地识别了TSS和TES,解决了转录组装中的一个关键挑战.
  • 泰洛斯有效地对组装成绩单进行排名,提高了整体组装质量.

结论:

  • 泰洛斯通过专注于精确的TSS和TES检测,在转录组装方面取得了重大进展.
  • 该框架是模块化的,可扩展的,并且与各种测序技术和汇编器兼容.
  • 预计Telos将在转录学研究中得到广泛采用,以更准确地进行基因表达分析.