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Updated: Jan 10, 2026

Identification of Post-translational Modifications of Plant Protein Complexes
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Identification of Post-translational Modifications of Plant Protein Complexes

Published on: February 22, 2014

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通过混合精密质谱法在激酶复合体中的蛋白形解析酸化动力学

Boris Krichel, Hsin-Ju Chan, Liam Bandura

    bioRxiv : the preprint server for biology
    |November 24, 2025
    PubMed
    概括

    这项研究引入了一种混合质谱法 (MS) 方法,用于分析酶复合体中的蛋白质酸化动态. 该方法揭示了AMP激活蛋白激酶 (AMPK) 中协调的自酸化级联和蛋白型模式.

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    Characterization at the Molecular Level using Robust Biochemical Approaches of a New Kinase Protein

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    A Mass Spectrometry-Based Approach to Identify Phosphoprotein Phosphatases and their Interactors10:17

    A Mass Spectrometry-Based Approach to Identify Phosphoprotein Phosphatases and their Interactors

    Published on: April 29, 2022

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    相关概念视频

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    Protein Kinases and Phosphatases

    Proteins undergo chemical modifications that trigger changes in the charge, structure, and conformation of the proteins. Phosphorylation, acetylation, glycosylation, nitrosylation, ubiquitination, lipidation, methylation, and proteolysis are various protein modifications that regulate protein activity. Such modifications are usually enzyme-driven.
    Protein kinases
    Many proteins in the cell are regulated by phosphorylation, the addition of a phosphate group. A family of enzymes called kinases...
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    Phosphorylation01:02

    Phosphorylation

    The addition or removal of phosphate groups from proteins is the most common chemical modification that regulates cellular processes. These modifications can affect the structure, activity, stability, and localization of proteins within cells as well as their interactions with other proteins.
    During phosphorylation, protein kinases transfer the terminal phosphate group of ATP to specific amino acid side chains of substrate proteins. Serine, threonine, and tyrosine are the most commonly...
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    Peptide Identification Using Tandem Mass Spectrometry01:33

    Peptide Identification Using Tandem Mass Spectrometry

    Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
    This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
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