前酸脱酶的进化适应:在生态利基专业化领域的监管ACT领域的收购
bioRxiv : the preprint server for biology
|November 24, 2025
概括
具有ACT域的细菌前酸脱酶 (PDH) 用氨酸进行全调节. 删除ACT域消除了这种调节,这表明其他因素有助于细菌的氨酸抑制.
科学领域:
- 生物化学 生物化学
- 分子生物学分子生物学
- 进化生物学 进化生物学
背景情况:
- 细菌前酸脱酶 (PDH) 对于氨酸生物合成至关重要.
- 来自Bacillaceae家族的PDH中的ACT域通过氨酸调节全调节,但机制尚不清楚.
- 讨论了ACT域介导的代谢调节的进化益处.
研究的目的:
- 为了研究由ACT域介导的PDH由氨酸的全调节机制.
- 确定ACT域在氨酸对PDH的调节作用中的作用.
- 探索在PDH中ACT域获取的进化影响.
主要方法:
- 生成和结构性分析缺少ACT域的截断PDH结构.
- 在氨酸的存在和缺席下评估截断的PDH的酶活性.
- 在含有ACT和缺乏ACT的PDH变体之间进行了比较结构分析.
- 对含有ACT的PDH分布进行了家族遗传分析.
主要成果:
- 缺少ACT域的截断PDH仍然具有催化活性,但失去了氨酸的全调节.
- 结构性比较显示了截断和自然缺乏ACT的PDH之间的前酸结合位循环中的轻微变化.
- 氨基酸替代并没有恢复对氨酸的敏感性,这表明远处的残留物参与其中.
- 含有ACT的PDH在Firmicutes和Actinomycetota中普遍存在,通常在极端环境中发现.
结论:
- 该ACT域对于氨酸对PDH的全调节至关重要.
- 氨酸抑制涉及超出基质结合部位和ACT域的残留物.
- 在特定细菌系中含有ACT的PDH的流行表明在营养有限的条件下具有进化优势.
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