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Updated: Jan 10, 2026

Massively Parallel Reporter Assays in Cultured Mammalian Cells
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Massively Parallel Reporter Assays in Cultured Mammalian Cells

Published on: August 17, 2014

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大规模并行记者测定揭示了转录起始点中的促进子,位置和链特异效应

Maya Bose1, Adelaide Tovar1, Yasuhiro Kyono1

  • 1Department of Computational Medicine and Bioinformatics, University of Michigan, Ann Arbor, MI 48109, USA.

bioRxiv : the preprint server for biology
|November 24, 2025
PubMed
概括

大规模并行报告员测试 (MPRA) 可以通过测试各种促进者和位置背景来揭示基因调控差异. 这种方法通过更全面地检查 cis-regulatory 元素来增强对复杂遗传疾病的理解.

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Promoter Capture Hi-C: High-resolution, Genome-wide Profiling of Promoter Interactions10:16

Promoter Capture Hi-C: High-resolution, Genome-wide Profiling of Promoter Interactions

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Live-Cell Imaging of Transcriptional Activity at DNA Double-Strand Breaks09:07

Live-Cell Imaging of Transcriptional Activity at DNA Double-Strand Breaks

Published on: September 20, 2021

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The Eukaryotic Promoter Region

The eukaryotic promoter region is a segment of DNA located upstream of a gene. It contains an RNA polymerase binding site, a transcription start site, and several cis-regulatory sequences.  The proximal promoter region is located in the vicinity of the gene and has cis-regulatory sequences and the core promoter. The core promoter is the binding site for RNA polymerase and is usually located between -35 and +35 nucleotides from the transcription start site. The distal promoter regions are...
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Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
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