通过转子子体-等离子体共同进化的群体级反控制的出现.
bioRxiv : the preprint server for biology
|November 24, 2025
概括
这项研究表明,细菌种群如何进化反控制. 移动遗传元素使大肠杆菌能够动态地对四环素等抗生素做出反应.
科学领域:
- 进化生物学是进化的生物学.
- 微生物遗传学微生物遗传学
- 系统生物学 系统生物学
背景情况:
- 适应性功能的起源是不太了解的.
- 大肠杆菌菌株与高复制等离子体被用来研究进化.
- 研究了对四环素的反应中的绿色光蛋白 (GFP) 表达.
研究的目的:
- 调查埃舍里奇亚大肠杆菌在人口水平反控制的新演变.
- 了解选择如何在单细胞内维持等离子体.
- 分析GFP表达,细菌健康和四环素反应之间的关系.
主要方法:
- 使用高复制等离子体的克隆大肠杆菌菌株.
- 引入了一个tetA-gfp四环素耐药性转体子.
- 与四环素和等离子体动力学相关的应用选择压力.
主要成果:
- 观察了人口层面反控制的 de novo 演变.
- 证明选择通过负反来维持tetA+和tetA-等离子体.
- 显示小突变可以导致人口行为的显著变化.
结论:
- 聚态细胞内等离子体种群的进化使宿主对抗生素的动态反应成为可能.
- 反控制机制可以在微生物群体中迅速演变.
- 这为理解适应性函数的演变提供了一个模型.
更多相关视频
15:00Daily Transfers, Archiving Populations, and Measuring Fitness in the Long-Term Evolution Experiment with Escherichia coli
Published on: August 18, 2023
4.2K
11:36Creation of a Dense Transposon Insertion Library Using Bacterial Conjugation in Enterobacterial Strains Such As Escherichia Coli or Shigella flexneri
Published on: September 23, 2017
16.6K
相关概念视频
Overview of Transposition and Recombination
18.7K
Transposons make up a significant part of genomes of various organisms. Therefore, it is believed that transposition played a major evolutionary role in speciation by changing genome sizes and modifying gene expression patterns. For example, in bacteria, transposition can lead to conferring antibiotic resistance. Movement of transposable elements within the genetic pool of pathogenic bacteria can aid in transfer of antibiotic-resistant genetic elements. In eukaryotes, transposons can carry out...
18.7K
Transposons
1.2K
Transposons, or "jumping genes," are small mobile genetic elements (MGEs) that range from 700 to 40,000 base pairs in length. They are found in all organisms and can move within the same chromosome or transfer to different chromosomes. In some cases, transposons can also jump between different host DNA molecules, such as plasmids or viruses, contributing to genetic variability.Barbara McClintock first discovered these mobile genetic elements in the 1940s while studying maize genetics, and she...
1.2K
Transduction
1.1K
Among the three main modes of HGT—transformation, conjugation, and transduction—transduction is unique in that it is mediated by bacteriophages, or bacterial viruses.Transduction occurs in two ways. Generalized transduction occurs during the lytic cycle of a bacteriophage infection. In this process, bacteriophages infect bacterial cells, replicate within them, and ultimately cause cell lysis, releasing newly assembled virions. Occasionally, random fragments of the bacterial genome...
1.1K
DNA-only Transposons
17.1K
DNA-only transposons are called autonomous transposons since they code for the enzyme transposase that is required for the transposition mechanism. Insertion of transposons can alter gene functions in multiple ways. They can mutate the gene, alter gene expression by introducing a novel promoter or insulator sequence, introduce new splice sites, and change the mRNA transcripts produced, or remodel chromatin structure.
The donor site from where the transposon is excised is either degraded or...
The donor site from where the transposon is excised is either degraded or...
17.1K
Gene Flow
37.3K
Gene flow is the transfer of genes among populations, resulting from either the dispersal of gametes or from the migration of individuals.
37.3K
Repressible Operon: trp Operon
1.3K
The trp operon in Escherichia coli exemplifies a repressible operon. It regulates the synthesis of tryptophan through repressor-mediated transcriptional control and attenuation. This dual regulatory mechanism ensures tryptophan biosynthesis occurs only when needed, conserving cellular resources.Structure of the trp OperonThe trp operon consists of five structural genes (trpE, trpD, trpC, trpB, and trpA) that encode enzymes for tryptophan biosynthesis. These genes are transcribed as a single...
1.3K
