在老鼠视觉皮层中,皮层-皮层连接的结构化和特定目标的发展
bioRxiv : the preprint server for biology
|November 24, 2025
概括
皮层投射模式是通过定向轴突准而形成的,而不是全球过度生长和修剪. 特定的神经元亚型很早就建立了精确的连接,在本地精细化,以便准确地连接大脑电线.
科学领域:
- 神经科学是一个神经科学.
- 发展生物学 发展生物学
- 系统神经科学 系统神经科学
背景情况:
- 哺乳动物皮层连接是刻板的,但精确投射模式的发育原理尚不清楚.
- 现有两种模式:早期的繁茂生长与修剪,或最初的定向轴突向.
研究的目的:
- 在小鼠中系统地绘制V1皮层-皮层投影神经元 (CCPNs) 到更高视觉区域 (HVA) 的产后发育.
- 解决目标特异性是否来自修剪或直接准.
主要方法:
- 使用快速和互补的逆行,逆行前和单细胞追踪方法.
- 采用多重逆行追踪和MAPseq进行单细胞分析.
主要成果:
- V1→HVA连接通过分阶段的轴突延伸和修剪来发展,与目标位置保持一致.
- 个别的V1CCPN很早就建立了特定的投影图案,变化有限.
- 双向V1-HVA连接逐渐完善,类似的连接在开发过程中对齐.
结论:
- V1→HVA连接通过定向轴突向形成,早期建立动机.
- 中间的目标是早些时候内化并逐渐精炼的;侧面的目标是晚些时候内化并迅速精炼.
- 送和反V1-HVA电路同时出现,并在整个开发过程中进行改进.
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