Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Alternative RNA Splicing02:18

Alternative RNA Splicing

24.6K
Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
24.6K
Alternative RNA Splicing02:18

Alternative RNA Splicing

4.8K
4.8K
RNA Splicing01:32

RNA Splicing

60.3K
Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
60.3K
Chromatin Structure Regulates pre-mRNA Processing02:41

Chromatin Structure Regulates pre-mRNA Processing

8.1K
In eukaryotic cells, nascent mRNA transcripts need to undergo many post-transcriptional modifications to reach the cell cytoplasm and translate into functional proteins. For a long time, transcription and pre-mRNA processing were considered two independent events that occur sequentially in the cell. However, it has now been well established that transcription and pre-mRNA processing are two simultaneous processes that are precisely regulated inside the cell.
The chromatin structure, especially...
8.1K
Regulation of Expression at Multiple Steps01:23

Regulation of Expression at Multiple Steps

1.3K
The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the...
1.3K
Pre-mRNA Processing: RNA Splicing01:36

Pre-mRNA Processing: RNA Splicing

6.5K
6.5K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

CDK8 Inhibition Releases the Muscle Differentiation Block in Fusion-driven Alveolar Rhabdomyosarcoma.

Cancer discovery·2026
Same author

Cognitive function depends upon <i>Satb2</i> gene dosage in cortical projection neurons.

bioRxiv : the preprint server for biology·2026
Same author

Type I interferon primes the alveolar epithelium to receive reparative signals from tissue-resident macrophages.

bioRxiv : the preprint server for biology·2026
Same author

Probing the limits of genetic recoding using multi-omics-guided evolution.

Nature communications·2026
Same author

Aging disrupts spatiotemporal coordination in the cycling murine ovary.

Nature aging·2026
Same author

P-glycoprotein-mediated efflux of rapaprotin-L dictates sensitivity of cancer cells to the inducer of 26S proteasome disassembly.

European journal of pharmacology·2026

相关实验视频

Updated: Jan 10, 2026

Engineering Artificial Factors to Specifically Manipulate Alternative Splicing in Human Cells
10:06

Engineering Artificial Factors to Specifically Manipulate Alternative Splicing in Human Cells

Published on: April 26, 2017

9.3K

针对可编程基因调节的细胞类型特定RNA分割元件的生成设计.

Xi Dawn Chen1,2,3, Maile Jim1,2,3, Mounica Vallurupalli1,4

  • 1Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA.

bioRxiv : the preprint server for biology
|November 24, 2025
PubMed
概括

研究人员开发了SPICE (细胞类型中的剪接比例),这是一个使用替代RNA剪接进行细胞类型特定基因控制的新框架. 这种方法使得可编程的基因调节在各种细胞类型的研究和治疗.

更多相关视频

A Reporter Based Cellular Assay for Monitoring Splicing Efficiency
08:53

A Reporter Based Cellular Assay for Monitoring Splicing Efficiency

Published on: September 15, 2021

3.2K
Using the E1A Minigene Tool to Study mRNA Splicing Changes
10:25

Using the E1A Minigene Tool to Study mRNA Splicing Changes

Published on: April 22, 2021

5.4K

相关实验视频

Last Updated: Jan 10, 2026

Engineering Artificial Factors to Specifically Manipulate Alternative Splicing in Human Cells
10:06

Engineering Artificial Factors to Specifically Manipulate Alternative Splicing in Human Cells

Published on: April 26, 2017

9.3K
A Reporter Based Cellular Assay for Monitoring Splicing Efficiency
08:53

A Reporter Based Cellular Assay for Monitoring Splicing Efficiency

Published on: September 15, 2021

3.2K
Using the E1A Minigene Tool to Study mRNA Splicing Changes
10:25

Using the E1A Minigene Tool to Study mRNA Splicing Changes

Published on: April 22, 2021

5.4K

科学领域:

  • 分子生物学分子生物学
  • 遗传学 遗传学 是一个
  • 生物信息学是一种生物信息学.

背景情况:

  • 控制特定细胞类型中的基因表达对于生物研究和开发新疗法至关重要.
  • 现有的细胞特异性基因调节方法在不同细胞类型的可扩展性和适用性方面面临挑战.

研究的目的:

  • 引入SPICE (细胞类型中的分离比例),这是一个可编程,细胞类型特定基因调节的新框架.
  • 利用替代RNA拼接作为一种精确控制基因表达的方法.
  • 为研究和治疗应用展示工程细胞类型特定拼接的潜力.

主要方法:

  • 开发了一种大规模并行报告测试 (MPRA) 用46,372个人类序列.
  • 从10个不同的血统中,对43个细胞系进行了表征性外显子跳跃模式.
  • 训练有素的深度学习模型来预测拼接,并通过编程的拼接模式生成合成序列.

主要成果:

  • 发现了广泛的细胞类型特定的替代RNA拼接事件.
  • 创建了能够预测新细胞环境中的拼接预测的预测模型.
  • 生成合成序列,表现出编程的,特定于细胞类型的拼接行为.
  • 工程序列可以在具有特定瘤突变的细胞中选择性地拼接.

结论:

  • SPICE提供了一个可扩展和可泛化的策略,用于剖析替代拼接法规.
  • 这种框架使得替代拼接的工程能够用于精确的基因表达控制.
  • 香料在促进基础生物研究和治疗干预方面都有很大的潜力.