Cln3可以独立于Whi5在起始酵母的细胞大小上工作
Amrita Shah1, Sangeet Honey1, Richard Sejour1
1Dept. of Microbiology and Immunology, Stony Brook University, Stony Brook, NY.
bioRxiv : the preprint server for biology
|November 24, 2025
概括
在酵母中,细胞分裂承诺 (Start) 受到CLN3和WHI5.5的调节. 这项研究发现CLN3功能独立于WHI5,挑战了以前的细胞大小控制模型.
科学领域:
- 细胞生物学 细胞生物学
- 遗传学 遗传学 是一个
- 分子生物学分子生物学
背景情况:
- 酵母细胞承诺在酵母细胞分裂时进行分裂.
- 开始 开始 开始
- 在G1阶段.
- 开始时的细胞大小是由CLN3和WHI5.5大小基因控制的.
- Cln3 激活了启动; Whi5 抑制了启动.
研究的目的:
- 为了研究细胞大小控制中CLN3和WHI5之间的关系.
- 为了确定CLN3和WHI5是否在线性路径中起作用.
主要方法:
- 同源性野生型,cln3,whi5,和cln3 whi5酵母突变体的比较.
- 对细胞大小和生长速度的分析.
主要成果:
- 这种whi5突变对CLN3并非完全表皮性,这表明CLN3可以独立于WHI5.5运作.
- 这种cln3 whi5双突变体表现出接近野生类型的细胞大小和受控的表型.
- 野生类型的细胞表现出最快的翻倍时间,这表明在野生类型的大小下最佳的生长率.
结论:
- 对CLN3和WHI5的线性通路模型没有完全支持.
- CLN3和WHI5是酵母细胞大小的关键调节者.
- 最佳的细胞大小与最快的生长率有关.
相关概念视频
Cells Coordinate Growth and Proliferation
5.0K
Cell size is a significant factor impacting cellular design, function, and fitness. There exists some internal coordination by which cells double their masses before division, thus, achieving homeostasis. Coordination between cell growth and proliferation depends on the checkpoints in between cell cycle phases. Loss of coordination or failure in the checkpoint mechanism can drive the cell to uncontrolled growth and loss of cellular function. Like dividing cells that coordinate cellular growth,...
5.0K
Yeast Signaling
17.1K
Yeasts are single-celled organisms, but unlike bacteria, they are eukaryotes (cells with a nucleus). Cell signaling in yeast is similar to signaling in other eukaryotic cells. A ligand, such as a protein or a small molecule released from a yeast cell, attaches to a receptor on the cell surface. The binding stimulates second-messenger kinases to activate or inactivate transcription factors that further regulate gene expression. Many of the yeast intracellular signaling cascades have similar...
17.1K
Positive Regulator Molecules
6.4K
Mitotic cell division results in daughter cells that exactly resemble the parent cell. However, errors in the DNA replication or distribution of genetic material may lead to genetic mutations that may be passed down to every new cell formed from the resulting abnormal cell. Propagation of such mutant cells is restricted through checkpoint mechanisms present at different stages of the cell cycle. These checkpoints involve regulator molecules that either promote or demote cell cycle events.
6.4K
Positive Regulator Molecules
133.9K
To consistently produce healthy cells, the cell cycle—the process that generates daughter cells—must be precisely regulated.
133.9K
Gene Regulation During Sporulation
420
Sporulation is a complex developmental process that allows certain Gram-positive bacteria, such as Bacillus subtilis and Clostridium species, to survive extreme environmental conditions. This process is tightly regulated by a series of signaling cascades and transcriptional controls, ensuring the formation of a highly resistant endospore.Sporulation is triggered by unfavorable conditions, such as nutrient depletion, and is governed by a phosphorelay system. One of the sensor kinases, such as...
420
The Cell Cycle Control System
13.9K
The cell cycle is an organized set of events that leads the cell to divide into two daughter cells, each containing chromosomes identical to the parent cell. It is the cell cycle that leads to the formation of an entire organism from a single-cell zygote. Besides, cell division also functions in the renewal or repair of tissues in adult multicellular eukaryotes. For example, in the bone marrow, the stem cells divide to form new blood cells. Although essential for several functions, cell...
13.9K


