流体液体:细胞和组织的DIY多重成像
John W Hickey1,2, Hongquan Li3,4, Chiara Caraccio2
1Department of Biomedical Engineering, Duke University, Durham, NC, USA.
bioRxiv : the preprint server for biology
|November 24, 2025
概括
流体-Squid是一个新的开源平台,用于单细胞和组织的成本效益高,可定制的多重成像. 这种自动流体学系统通过降低成本和提高适应性来增强细胞生物学研究.
科学领域:
- 细胞生物学 细胞生物学
- 生物技术是生物技术.
- 显微镜的使用方法
背景情况:
- 多复合单细胞表征提供了深刻的生物学见解,但往往受到高成本和有限的可定制性的阻碍.
- 现有的技术难以平衡可负担性与细胞生物学定制实验工作流的需求.
研究的目的:
- 开发Fluid-Squid,这是一个开源的,具有成本效益和可定制的自动流体平台,用于多重成像.
- 用高复合抗体面板来证明平台在单细胞和组织样本的特征化能力.
- 引入新的条形码策略,用于组合样本分析,减少实验时间,成本和批量效应.
主要方法:
- 开发Fluid-Squid,一个集成的自动流体学和显微镜系统.
- 用于高复数成像 (36复数和39复数) 的寡核酸条形抗体面板的应用.
- 实施样本聚合和条形码策略,用于同时分析多个样本.
主要成果:
- 使用Fluid-Squid成功对人类肠道组织进行成像,并对免疫细胞 (脊髓细胞,PBMCs) 进行表征.
- 对多重复合抗体面板的冷化可行性的证明.
- 针对抗体定位和血液制备方法的评估,聚合样本分析的验证.
结论:
- 流体液体为先进的多重成像提供了一个灵活,经济高效的开源框架.
- 该平台为研究人员提供了可适应的解决方案,用于单细胞和组织的表征.
- 这项工作显著降低了复杂的多重成像实验的进入壁垒.
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