来自hAT超级家族的多重巴斯特转位子在哺乳动物细胞中显示转位活动
Zhongxia Guan1, Binqing Wang1, Bo Gao1
1College of Animal Science & Technology, Yangzhou University, Yangzhou, Jiangsu, 225009, China.
Synthetic and systems biotechnology
|November 24, 2025
概括
研究人员从巴斯特 (BT) 转体子中发现了新的基因转移工具. 阿尔马迪利纳萨图姆巴斯特 (AnBT) 转基因酶显示出显著的转基因活性,使其成为基因治疗和转基因应用的有希望的候选者.
科学领域:
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
- 生物信息学是一种生物信息学.
背景情况:
- 修改后的巴斯特转子TcBuster是一种有效的基因转移工具.
- 超级hAT家族包括巴斯特转子子家族.
- 基因传递载体对于基因治疗至关重要.
研究的目的:
- 通过分析巴斯特转子子来发现新的基因转移工具.
- 为了评估新发现的巴斯特转位酶的转位活动.
- 评估这些转子体在基因治疗和转基因发生的潜力.
主要方法:
- 在Actinopterygii和Arthropoda中对巴斯特转位子进行生物信息分析.
- 基于细胞的转化试验测量转化酶活性.
- 确定转子子活动的关键终端序列.
- 帮助者和捐赠者等离子体度的优化,以获得最大的活性.
主要成果:
- 生物信息分析揭示了Actinopterygii和Arthropoda中的活跃的巴斯特转位子.
- 来自Armadillidium nasatum (AnBT),Hyles vespertilio (HvBT) 和Lota lota (LlBT) 的内源转化酶显示出显著的转化活性 (47%,44%,39%相对于piggyBac).
- 与piggyBac相比,AnBT转移酶显示出63%的转移效率,最佳活性在50 ng辅助等离子体和特定终端序列 (240 bp左,126 bp右) 中至关重要.
结论:
- AnBT转移酶是一种高度活跃的转移酶,具有作为基因传递载体的潜力.
- 鉴定到的巴斯特转子子扩大了基因治疗和转基因发生的工具包.
- 对AnBT的进一步研究可能会导致改进的基因转移策略.
更多相关视频
10:58Transposon Mediated Integration of Plasmid DNA into the Subventricular Zone of Neonatal Mice to Generate Novel Models of Glioblastoma
Published on: February 22, 2015
13.4K
04:04Real-Time Quantification of the Effects of IS200/IS605 Family-Associated TnpB on Transposon Activity
Published on: January 20, 2023
2.7K
相关概念视频
DNA-only Transposons
17.1K
DNA-only transposons are called autonomous transposons since they code for the enzyme transposase that is required for the transposition mechanism. Insertion of transposons can alter gene functions in multiple ways. They can mutate the gene, alter gene expression by introducing a novel promoter or insulator sequence, introduce new splice sites, and change the mRNA transcripts produced, or remodel chromatin structure.
The donor site from where the transposon is excised is either degraded or...
The donor site from where the transposon is excised is either degraded or...
17.1K
Transposons
1.2K
Transposons, or "jumping genes," are small mobile genetic elements (MGEs) that range from 700 to 40,000 base pairs in length. They are found in all organisms and can move within the same chromosome or transfer to different chromosomes. In some cases, transposons can also jump between different host DNA molecules, such as plasmids or viruses, contributing to genetic variability.Barbara McClintock first discovered these mobile genetic elements in the 1940s while studying maize genetics, and she...
1.2K
Overview of Transposition and Recombination
18.7K
Transposons make up a significant part of genomes of various organisms. Therefore, it is believed that transposition played a major evolutionary role in speciation by changing genome sizes and modifying gene expression patterns. For example, in bacteria, transposition can lead to conferring antibiotic resistance. Movement of transposable elements within the genetic pool of pathogenic bacteria can aid in transfer of antibiotic-resistant genetic elements. In eukaryotes, transposons can carry out...
18.7K
Non-LTR Retrotransposons
13.1K
As the name suggests, non-LTR retrotransposons lack the long terminal repeats characteristic of the LTR retrotransposons. Additionally, both LTR and non-LTR retrotransposons use distinct mechanisms of mobilization. Non-LTR retrotransposons are further divided into two classes - Long interspersed nuclear elements (LINEs) and short interspersed nuclear elements (SINEs), both of which occur abundantly in most mammals, including humans. Some of the active non-LTR retrotransposons in humans are L1...
13.1K
LTR Retrotransposons
19.4K
LTR retrotransposons are class I transposable elements with long terminal repeats flanking an internal coding region. These elements are less abundant in mammals compared to other class I transposable elements. About 8 percent of human genomic DNA comprises LTR retrotransposons. Some of the common examples of LTR retrotransposons are Ty elements in yeast and Copia elements in Drosophila.
The internal coding region of LTR retrotransposons and their mechanism of transposition closely resembles a...
The internal coding region of LTR retrotransposons and their mechanism of transposition closely resembles a...
19.4K
piRNA - Piwi-interacting RNAs
7.5K
PIWI-interacting RNAs, or piRNAs, are the most abundant short non-coding RNAs. More than 20,000 genes have been found in humans that code for piRNAs while only 2000 genes have been found for miRNAs. piRNAs can act at the transcriptional and post-transcriptional levels and have a vital role in silencing transposable elements present in germ cells. They are also involved in epigenetic silencing and activation. Previously, they were thought to function only in germ cells but new evidence suggests...
7.5K
