使用微生物组分析作为临床试验中的补充终点
Lourdes Velo-Suarez1, Charles-Antoine Guilloux2, Rozenn Le Berre2,3
1Center for Biome Analysis and Microbiota (CBAM), CHU Brest, 29200 Brest, France.
iScience
|November 24, 2025
概括
在囊性纤维化患者中,R-roscovitine治疗对Pseudomonas aeruginosa没有直接影响,但改变了肠道和肺部微生物组. 该药物以剂量依赖的方式影响了微生物社区结构,表明了个性化的微生物组效应.
科学领域:
- 微生物学 微生物学
- 药理学 药理学 是一个学科.
- 临床医学 临床医学
背景情况:
- 囊性纤维化 (CF) 患者经常经历慢性 Pseudomonas aeruginosa 殖民.
- 了解除了直接向病原体之外的治疗效应,对于CF管理至关重要.
- 肠道和肺部的微生物组在CF的发病和进展中起着重要的作用.
研究的目的:
- 评估口服R-roscovitine在CF患者的安全性和有效性.
- 调查R-roscovitine对肺部和肠道微生物群落的影响.
- 探索R-roscovitine对微生物组结构和动态的剂量依赖作用.
主要方法:
- 罗斯科-CF研究涉及口服R-罗斯科维给CF患者.
- 在治疗前和治疗后收集了唾液和便样本.
- 使用16S rDNA测序来分析微生物社区的组成和多样性.
主要成果:
- R-roscovitine并没有直接影响Pseudomonas aeruginosa的殖民活动.
- 微生物组的α多样性保持稳定,但β多样性表现出剂量依赖的变化.
- 特定细菌种群的丰度随着R-roscovitine剂量变化,Tannerella和Granulicatella elegans的增加,Streptococcus的减少.
结论:
- R-roscovitine可能以个性化,剂量依赖的方式调节肠道和肺部的微生物组.
- 微生物组分析可以作为CF临床试验中的一个有价值的探索性终点.
- 需要进一步的研究来阐明这些微生物组变化的临床影响.
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