相关实验视频
Updated: Jan 10, 2026
09:30
Ammonia Fiber Expansion AFEX Pretreatment of Lignocellulosic Biomass
Published on: April 18, 2020
14.3K
玉米废物的糖化和乙醇生产使用当地生产的Trichoderma atroviride酶合尾酒
Mariana Fornazier Borges1, Geraldo Aparecido da Rocha Junior2, Daniel Pasquini1
1Federal University of Uberlândia, Chemical Institute (IQ-UFU), Uberlândia, Minas Gerais 38400-902, Brazil.
ACS omega
|November 24, 2025
概括
来自Trichoderma atroviride的自制酶尾酒有效地将玉米和干糖化为纤维素乙醇. 添加Tween 20表面活性剂显著增加了糖的释放,提高了发酵产量.
科学领域:
- 生物技术是生物技术.
- 酶学 是一种酶学.
- 可再生能源可再生能源是可再生能源.
背景情况:
- 纤维素生物质,如玉米和,是纤维素乙醇生产的有希望的原料.
- 有效的酶解水解对于最大限度地提高从纤维素酸中获取糖的产量至关重要.
- 开发具有成本效益的酶溶液对于生物燃料的经济可行性至关重要.
研究的目的:
- 为了评估Trichoderma atroviride自制的酶尾酒,用于玉米和的糖化.
- 为了优化水解条件,使用料批量模式和表面活性剂添加.
- 评估从经过处理的生物质中生产纤维素乙醇的潜力.
主要方法:
- 固态发酵Trichoderma atroviride产生一个富含细胞酶和半细胞酶的酶尾酒.
- 玉米和玉米的性预处理.
- 用自制尾酒进行Feed-batch酶化水解,含有或不含Tween 20表面活性剂.
- 对释放的总减少糖 (TRS) 的分析.
- 化物的发酵以确定理论转换产量.
主要成果:
- 酶尾酒显示了高的β-葡萄糖酶 (374.91 U/g) 和西兰酶 (920 U/g) 活性.
- β-葡萄糖酶的最佳条件是pH值为4.5-5.0和50°C.
- 用预处理的玉米 (PTCC) 的料批量水解产生了15.86g/L的TRS.
- 添加1%的Tween 20增加了PTCC水解中的TRS,达到30.37g/L (91.4%的增加).
- 酒精发酵实现了从PTCC水解液的83.48%的理论转换产量.
结论:
- 特里科德玛 (Trichoderma atroviride) 是一种可行的低成本来源,用于生产有效的酶式尾酒.
- 料批量水解和表面活性剂添加的组合显著提高了糖化效率.
- 这种方法证明了从玉米和玉米中生产纤维素乙醇的可行性.
相关概念视频
01:20Fates of Pyruvate
10.4K
Pyruvate is the end product of glycolysis, where glucose is oxidized to pyruvate, simultaneously reducing NAD+ to NADH. Two molecules of ATP are also produced by substrate-level phosphorylation.
In aerobic organisms, pyruvate is metabolized via the citric acid cycle to produce reduced coenzymes NADH and FADH2. These coenzymes are then oxidized in the electron transport chain to produce ATP and, in the process, regenerate the NAD+ and FAD. As seen in some cell types and organisms, fermentation...
In aerobic organisms, pyruvate is metabolized via the citric acid cycle to produce reduced coenzymes NADH and FADH2. These coenzymes are then oxidized in the electron transport chain to produce ATP and, in the process, regenerate the NAD+ and FAD. As seen in some cell types and organisms, fermentation...
10.4K
01:23Microbial Fermentation
1.3K
Fermentation is a crucial anaerobic metabolic process that enables microbes to derive energy from sugar without relying on oxygen or an electron transport chain. This process is fundamental to various biological and industrial applications and is classified based on the metabolic products generated.Role of Pyruvate in FermentationPyruvate and its derivatives serve as key electron acceptors in fermentative pathways. The oxidation of NADH to regenerate NAD+ is essential for the continuation of...
1.3K