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黄金纳米颗粒SERS在尿液中的狼患者的炎诊断中的作用

Alberto Carlos Espinosa^1,2, Alejandra Rojo-Sanchez¹, Ada Carmona-Martes¹

¹Facultad de Ciencias Básicas y Biomédicas, Life Science Research Center, Universidad Simón Bolívar, Barranquilla 080002, Colombia.

|November 24, 2025

概括

这项研究引入了一种使用SERS和黄金纳米颗粒检测系统性红斑狼 (SLE) 患者狼神经炎 (NL) 的新型非侵入性尿液测试. 该方法的准确性很高,有可能使早期诊断和监测这种严重并发症.

科学领域:

生物医学工程生物医学工程
分析化学分析化学
免疫学免疫学免疫学

背景情况:

系统性红斑狼 (SLE) 是一种自身免疫性疾病,在这种疾病中,狼性炎 (NL) 显著增加了发病率和死亡率.
脏活检,目前NL诊断的标准,是侵入性的,不适合频繁监测.
对于早期NL检测和监测而言,极少侵入性,准确的方法至关重要.

研究的目的:

通过尿液分析开发和验证狼性炎的快速,最少侵入性诊断工具.
为了区分健康对照组,没有炎的SLE患者和经活检确认的NL患者.
评估使用开发的方法早期检测NL进展的潜力.

主要方法:

表面增强的拉曼光谱法 (SERS) 用于尿样,用酸盐减少黄金纳米粒子 (AuNPs) 增强.
部分最小平方区分分析 (PLS-DA) 用于分析来自235个尿样 (72个对照组,70个SLE,93个NL) 的SERS光谱.
用交叉验证和外部预测评估模型性能,测量灵敏度,特异性和准确性.

主要成果:

SERS-PLS-DA模型实现了高诊断准确性,C与NL模型通过交叉验证显示了96.8%的准确性,通过外部预测显示了97.6%.
分析确定了关键的拉曼带,与尿素,肌素,氨基酸和脂质等代谢物相关,表明NL的代谢途径受到干扰.
对三名患者的纵向分析显示,SERS-PLS-DA管道在临床诊断前两年检测到NL特征.

结论:

尿液的AuNP增强SERS,加上化学计量建模,为区分SLE表型提供了一个灵敏,经济有效和非侵入性的平台.
这种方法可以早期,非侵入性检测狼性炎,可能在临床表现之前几年.
在未来的队列中进一步部署可以促进常规NL查,并指导SLE患者及时进行治疗干预.

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