低成本,无标签的电化学感应器,用于双重检测登革热和寨卡病毒,使用化氧化改性APTES和金纳米粒子
Bassam Bachour Junior1, Marina Ribeiro Batistuti Sawazaki2, Ricardo Estéfani França Rocha1
1Department of Physics, Faculty of Philosophy, Sciences and Letters, University of São Paulo, 14040-901 Ribeirão Preto, São Paulo, Brazil.
ACS omega
|November 24, 2025
概括
这项研究开发了一种负担得起的生物传感器,用于检测登革热和寨卡病毒感染. 该平台在资源有限的环境中显示出对同时进行 arbovirus 诊断的前景.
科学领域:
- 生物材料科学 生物材料科学
- 纳米技术纳米技术
- 生物传感器开发开发
背景情况:
- 全球范围内,登革热和寨卡病毒等 arbovirus 感染病例正在增加,特别是在热带和资源较低的地区.
- 对于这些疾病来说,急需可访问和快速诊断的工具.
研究的目的:
- 设计一个具有成本效益的平台,用于同时检测登革热和寨卡病毒生物标志物.
- 为了优化和验证开发的 aptasensor.to 的性能.
主要方法:
- 用黄金纳米颗粒和DNA吸收剂修改的氧化基质的制造.
- 利用电化学阻抗光谱学用于apt传感器的表征和性能评估.
- 采用非结构性蛋白1 (NS1) 作为登革热和寨卡病毒的目标生物标志物.
主要成果:
- 生物传感器在PBS中实现了登革热0.21 ng/mL的检测极限 (LoD),在人血清中达到0.55 ng/mL.
- 在登革热检测方面表现出高灵敏度,在PBS中为每十年19.26%,在血清中为每十年11.87%.
- 在PBS中检测寨卡病毒的LoD为0.68 ng/mL,每十年的灵敏度为26.34%.
结论:
- 开发的食感器平台可以同时检测登革热和寨卡病毒,为临床诊断提供了潜在的解决方案.
- 虽然无法区分这两种病毒,但该平台的可负担性和有效性对资源有限的环境来说是有希望的.
- 需要进一步的研究来解决像人类血清这样复杂的生物矩阵中的非线性传感器反应.
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