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结直肠癌中的微RNA:循环和组织微RNA水平的比较分析

Iulia Andreea Pelisenco¹, Bogdan Trandafir², Anastasia-Maria Dobre²

¹Department of Pathology, Victor Babes National Institute of Pathology, Bucharest 050096, Romania.

World journal of gastrointestinal oncology

|November 24, 2025

概括

五种microRNA显示为检测结直肠癌 (CRC) 的非侵入性生物标志物具有前途. 这些循环的microRNAs可以增强早期查和个性化CRC的患者护理.

科学领域:

在瘤学瘤学.
分子生物学分子生物学
生物标志物发现发现

背景情况:

结肠直肠癌 (CRC) 是一种普遍存在的全球恶性瘤,需要改进早期检测方法.
目前的查依赖于结肠镜和便检测,但需要最小侵入性的生物标志物.
微RNAs (miRNAs) 正因为它们的稳定性和在癌症中的作用而成为潜在的生物标志物.

研究的目的:

为了确定循环中的microRNAs (miRNAs) 作为潜在的结直肠癌 (CRC) 生物标志物.
为了比较CRC患者瘤组织和血中的miRNA表达模式.
为了验证潜在的miRNA生物标志物用于临床应用.

主要方法:

案例控制研究的设计.
定量实时聚合酶连锁反应 (qRT-PCR) 分析了19名CRC患者瘤/周围瘤组织中的84个miRNA和血中的179个miRNA.
包括组织和血分析的对照队列.

主要成果:

14个miRNA在组织和血样本中表现出显著的差异性表达.
五个特定的miRNAs (miR-26b-5p,miR-101-3p,miR-30d-5p,miR-107,miR-21-5p) 在样本类型中显示出一致的表达趋势.
循环中的miR-21-5p和miR-26b-5p水平与CRC患者的淋巴血管侵入有关.

结论:

已识别的五种miRNA是结直肠癌 (CRC) 的潜在非侵入性生物标志物.
这些生物标志物可以提高CRC查效率.
它们可能支持开发精准医学和个性化的CRC患者管理策略.

关键词:

生物标志物生物标志物结肠粘膜中的结肠粘膜.结肠直肠癌是一种癌症.淋巴血管侵入淋巴血管侵入微RNAs是一种微RNA.在等离子体中,我们可以得到等离子体.

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相关概念视频

MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...

MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...