使用转录组学解读日本脑炎病毒的核心遗传和通路签名
Mohd Ali1, Mohd Shawaz1, Noor Saba Khan2
1Department of Pharmacology, Ram-Eesh Institute of Vocational and Technical Education, Greater Noida, UP 201310 India.
Virusdisease
|November 24, 2025
概括
这项研究确定了参与日本脑炎病毒 (JEV) 感染的关键基因. 这些已识别的枢纽基因为开发针对JEV的新疗法提供了潜在的治疗点.
科学领域:
- 病毒学 病毒学
- 生物信息学是一种生物信息学.
- 基因组学就是基因组学.
背景情况:
- 日本脑炎病毒 (JEV) 是一种在亚洲普遍存在的蚊子传播的动物性病原体.
- 热血病病毒会导致严重的神经损伤和死亡,目前还没有专门的治疗方法可用.
- 了解JEV的分子机制对于治疗开发至关重要.
研究的目的:
- 用生物信息学来确定JEV治疗干预的潜在分子标.
- 在JEV感染中分析差异表达基因 (DEGs).
- 为了发现新的生物标志物和治疗策略对JEV.
主要方法:
- 使用生物信息学来分析NCBI-GEO资料中的基因表达数据 (GSE39740,GSE57330,GSE115167).
- 使用R simpleaffy识别差异表达基因 (DEGs);使用clusterProfiler进行基因本体学和通路丰富分析.
- 使用Cytoscape构建基因网络,使用MCODE和CytoHubba识别中心基因.
主要成果:
- 在JEV感染中确定了1,601个DEGs (1,103个上调,498个下调).
- 发现了37个枢纽基因,其中ACTB,ALB,CASP3,CDK4,CXCL10,CXCL8,CXCR4,DDX58,DICER1和EGR1是前十名.
- ACTB被确定为最重要的枢纽基因,其高连接度为243.
结论:
- 已识别的枢纽基因是JEV的有希望的治疗点.
- 这项研究增强了对受到JEV影响的分子通路的理解.
- 结果为开发JEV生物标志物和治疗方法提供了基础,以减少全球健康影响.
相关概念视频
01:29Viruses with RNA Genomes
765
RNA viruses are categorized into positive-strand, negative-strand, or double-stranded groups based on their genomic structure and replication mechanisms. This classification dictates how they exploit host cellular machinery for protein synthesis and replication. Some RNA viruses also utilize reverse transcription as part of their life cycle, further diversifying their replication strategies.Positive-Strand RNA VirusesPositive-strand RNA viruses have genomes that function directly as messenger...
765
02:02Genomics
39.6K
Genomics is the science of genomes: it is the study of all the genetic material of an organism. In humans, the genome consists of information carried in 23 pairs of chromosomes in the nucleus, as well as mitochondrial DNA. In genomics, both coding and non-coding DNA is sequenced and analyzed. Genomics allows a better understanding of all living things, their evolution, and their diversity. It has a myriad of uses: for example, to build phylogenetic trees, to improve productivity and...
39.6K
02:34DNA Microarrays
20.6K
Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...
20.6K
02:15Experimental RNAi
7.2K
RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
7.2K
00:58Genomic DNA in Eukaryotes
52.2K
Eukaryotes have large genomes compared to prokaryotes. To fit their genomes into a cell, eukaryotic DNA is packaged extraordinarily tightly inside the nucleus. To achieve this, DNA is tightly wound around proteins called histones, which are packaged into nucleosomes that are joined by linker DNA and coil into chromatin fibers. Additional fibrous proteins further compact the chromatin, which is recognizable as chromosomes during certain phases of cell division.
52.2K
02:24Ribosome Profiling
4.0K
Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
4.0K