不同的尿液保存方法和添加防腐剂对24小时尿蛋白的定量检测的影响
Xinxin Bao1, Xiaoye Sun2, Haiying Geng1
1Department of Clinical Laboratory, Affiliated Maternity and Child Health Care Hospital of Nantong University, Nantong, China.
Frontiers in medicine
|November 24, 2025
概括
添加烯作为防腐剂不会影响24小时尿蛋白量化. 在没有防腐剂的情况下在室温下储存尿样是临床使用的安全有效方法.
科学领域:
- 临床化学 临床化学
- 生物化学 生化学
- 泌尿器科 泌尿器科 泌尿器科 泌尿器科
背景情况:
- 准确的24小时尿蛋白量化对于诊断脏疾病至关重要.
- 防腐剂通常用于在储存期间保持样品完整性,但它们对蛋白质水平的影响尚未完全理解.
- 研究具有成本效益和简单的储存方法对于广泛的临床应用至关重要.
研究的目的:
- 为了确定烯作为防腐剂对24小时尿蛋白测量的影响.
- 评估不同储存温度对含有或不含防腐剂的尿蛋白量化的影响.
- 在各种存储条件下评估蛋白尿检测的诊断准确性.
主要方法:
- 收集了80个尿样,蛋白质检测结果呈阳性.
- 在37°C,24-26°C和4°C的温度下存储含有和不含西的样本.
- 使用单向ANOVA分析蛋白质度,并通过ROC分析评估诊断准确度.
主要成果:
- 在所有测试温度中,在含有和不含烯的样本之间没有观察到24小时尿蛋白度的统计学上显著差异.
- 接收器操作特征 (ROC) 分析表明蛋白尿检测的诊断准确性很好 (AUC: 0.992-0.994).
- 没有防腐剂储存的尿样显示了可比的准确性,具有完美的特异性 (100%) 和高灵敏性 (97.4%),在4°C.
结论:
- 烯防腐剂不会显著改变24小时尿蛋白量化.
- 在没有防腐剂的情况下在室温下储存24小时的尿蛋白样本是一种可靠和实用的方法.
- 这种简化存储方法适合在临床环境中广泛实施.
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