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单核转录学揭示了小鼠大肠杆菌 (Escherichia coli) 发炎期间的性别差异

David Hunstad¹, Teri Hreha², Abigail Manson³

¹Washington University in St. Louis.

Research square

|November 24, 2025

概括

尿路感染 (UTI) 的性别差异显示出不同的脏反应. 男性表现出更广泛的细胞类型参与和损伤通路倾向,而女性在尿路感染期间表现出更集中的炎症反应.

科学领域:

免疫学免疫学免疫学
基因组学就是基因组学.
泌尿器科泌尿器科泌尿器科泌尿器科

背景情况:

尿路感染 (UTI) 在患病率和结果方面存在显著的性别差异.
与女性相比,男性患上上道尿路感染的发病率和死亡率较高.
临床前模型显示,暴露于雄性激素的雄性和雌性容易发生严重的炎症.

研究的目的:

为了调查性差异性细胞和分子反应在高位铁炎期间脏.
为了生成第一个整体脏单核转录基因数据集的脏感染.

主要方法:

单核RNA测序 (snRNA-seq) 在雌性,雄性和雄性暴露雌性小鼠的脏上进行了测序.
对照组接受了酸盐缓冲盐水 (PBS) 接种.
生物信息分析确定了细胞群和转录性变化.

主要成果:

在多种细胞类型中观察到性别不一致的转录反应.
女性的尿路感染反应涉及更少的细胞类型,具有高调的促炎基因.
雄性小鼠表现出对损伤途径的倾向,并且在更多细胞类型中作出反应,即使在盐水控制下也是如此.

结论:

脏感染引起了不同的性别特异性转录反应.
了解这些性别差异对于解决尿路感染结果差异至关重要.
生成的数据集为进一步对脏感染反应进行细胞和分子剖析提供了基础.

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