Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Cell-matrix's Response to Mechanical Forces01:13

Cell-matrix's Response to Mechanical Forces

3.4K
In animal cells, the extracellular matrix allows cells within tissues to withstand external stresses and transmits signals from the outside of the cell to the inside. The extracellular matrix is extensive, and its composition varies between different types of tissues. For example, the reticular fibers and ground substance make up the ECM in loose connective tissue, while collagen and bone minerals make up the ECM of bone tissue. 
Anchoring junctions mechanically attach a cell to the...
3.4K
Mechanically-gated Ion Channels01:12

Mechanically-gated Ion Channels

7.5K
Mechanically-gated ion channels are proteins found in eukaryotic and prokaryotic cell membranes that open in response to mechanical stress. Tension, compression, swelling, and shear stress can alter the conformation of the protein, opening a transmembrane channel that allows the passage of ions for signal transmission. In eukaryotes, mechanically-gated channels are distributed in several regions like the neurons, lungs, skin, bladder, and heart, where they play critical roles in numerous...
7.5K
Tension Response at Adherens Junctions01:26

Tension Response at Adherens Junctions

3.4K
The adherens junctions that anchor cells together are multi-protein complexes that dynamically adapt to mechanical stimuli such as tensile forces and shear stress. Mechanosensory proteins in these junctions can sense such mechanical stimuli and undergo a shift in their conformation, resulting in an altered function — a process called mechanotransduction.
α-Catenin as a Mechanosensory Protein
The α-catenin of adherens junctions is an allosteric protein with three VH (vinculin...
3.4K
Mechanism of Ciliary Motion01:05

Mechanism of Ciliary Motion

4.8K
The ciliary structures were first seen in 1647 by Antonie Leeuwenhoek while observing the protozoans. In lower organisms, these appendages are responsible for cell movement, while in higher organisms, these appendages help in the movement of the extracellular fluids within the body cavities.
The cilia are made up of microtubules in a 9+2 arrangement, with nine microtubule doublet ring bundles, surrounding a pair of central singlet microtubule bundles. The doublet microtubule bundles are...
4.8K
Mechanism of Lamellipodia Formation01:31

Mechanism of Lamellipodia Formation

3.5K
Cells migrating in response to external stimuli form lamellipodia, which are thin membrane protrusions supported by a mesh of linked, branched, or unbranched actin filaments. These actin filaments interact with myosin motor proteins, creating the dynamic actomyosin complex within the cytoskeleton. Contractility, or the ability to generate contractile stress, is inherent to the actomyosin complex. It helps cells detect the stiffness of the surrounding ECM and exert contractile force for...
3.5K
Mechanical Protein Functions01:58

Mechanical Protein Functions

5.5K
Proteins perform many mechanical functions in a cell. These proteins can be classified into two general categories- proteins that generate mechanical forces and proteins that are subjected to mechanical forces. Proteins providing mechanical support to the structure of the cell, such as keratin, are subjected to mechanical force, whereas proteins involved in cell movement and transport of molecules across cell membranes, such as an ion pump, are examples of generating mechanical force. 
5.5K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

An automated, digital immunoassay on a microfluidic cartridge for on-demand cytokine profiling.

Microsystems & nanoengineering·2026
Same author

An immunocompetent bone marrow-on-a-chip model for studying human hematological malignancies and preclinical therapeutic screening.

Nature protocols·2026
Same author

Shifting risk profiles in the systemic-to-focal transition of brucellosis: a multicenter analysis of spondyloarthritis development.

BMC infectious diseases·2026
Same author

Sensory neurons inhibit invadopodia and metastasis via direct CGRP-RAMP1-cAMP signaling to cancer cells.

bioRxiv : the preprint server for biology·2026
Same author

A Human Lymph node-on-a-Chip for Personalized Evaluation of Vaccine Immunogenicity.

Research square·2026
Same author

Ultrasound-guided HUC-MSCs transplantation alleviates neuropathic pain in CCI rats: a mechanistic study based on microglia/macrophage polarization and the NLRP3 inflammasome.

International immunopharmacology·2026

相关实验视频

Updated: Jan 10, 2026

DNA Tension Probes to Map the Transient Piconewton Receptor Forces by Immune Cells
06:53

DNA Tension Probes to Map the Transient Piconewton Receptor Forces by Immune Cells

Published on: March 20, 2021

3.1K

Piezo1介导的机能学调节了CAR T细胞的功能.

Ngoc Luu1, Rui Li2, Yifei Fang1

  • 1Department of Biomedical Engineering, Tandon School of Engineering, New York University, Brooklyn, NY 11201, USA.

Research square
|November 24, 2025
PubMed
概括

杀死CAR T细胞需要大量的机械力. 这项研究表明,Piezo1通道将机械力与细胞能量产生联系起来,优化CAR T细胞的功效,并为癌症免疫治疗提供了一种新策略.

更多相关视频

Spatial and Temporal Control of T Cell Activation Using a Photoactivatable Agonist
07:48

Spatial and Temporal Control of T Cell Activation Using a Photoactivatable Agonist

Published on: April 25, 2018

6.6K
One-channel Cell-attached Patch-clamp Recording
13:07

One-channel Cell-attached Patch-clamp Recording

Published on: June 9, 2014

25.3K

相关实验视频

Last Updated: Jan 10, 2026

DNA Tension Probes to Map the Transient Piconewton Receptor Forces by Immune Cells
06:53

DNA Tension Probes to Map the Transient Piconewton Receptor Forces by Immune Cells

Published on: March 20, 2021

3.1K
Spatial and Temporal Control of T Cell Activation Using a Photoactivatable Agonist
07:48

Spatial and Temporal Control of T Cell Activation Using a Photoactivatable Agonist

Published on: April 25, 2018

6.6K
One-channel Cell-attached Patch-clamp Recording
13:07

One-channel Cell-attached Patch-clamp Recording

Published on: June 9, 2014

25.3K

科学领域:

  • 免疫学 免疫学 免疫学
  • 细胞生物学 细胞生物学
  • 生物物理学的生物物理.

背景情况:

  • 卡尔-T细胞疗法的疗效依赖于机械力产生.
  • 细胞代谢与CAR T细胞中的机械转导之间的联系还不清楚.
  • 代谢重编程对于CAR T细胞效应器功能至关重要.

研究的目的:

  • 研究单个CAR T细胞中机械能耗和代谢状态之间的关系.
  • 在CAR T细胞中确定连接机械转导和代谢重编程的分子机制.
  • 探索Piezo1作为增强CAR T细胞功能的潜在治疗点.

主要方法:

  • 在单个CAR T细胞中直接测量突触力和机械能量.
  • 与机械测量相结合的代谢状态的分析.
  • 研究涉及Piezo1.1的Ca2+-Wnt-Rac1信号通路.
  • 在患者衍生和耗尽的细胞中评估CAR T细胞功能.

主要成果:

  • 机械能量效率与CAR T细胞细胞毒性强度直接相关.
  • 机械敏感的离子通道Piezo1将细胞骨动力学与代谢重新连接相结合.
  • 皮埃佐1的干扰会损害ATP的产生,导致能量压力和细胞毒性降低.
  • 一个中等水平的Piezo1活动优化了CAR T细胞的功能,而极端水平导致功能障碍.

结论:

  • 机械代谢合是CAR T细胞适应性的关键调节者.
  • Piezo1在将机械力与细胞代谢和功能联系起关键作用.
  • 调Piezo1活动为改进基于CAR T细胞的癌症免疫疗法提供了一种新的策略.