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青酸形状微生物组组成在中肠和培养小宇宙的Aedes triseriatus (说)
Shicheng Chen1, Liang Cui2, Bin Zuo3
1Medical Laboratory Science, College of Health and Human Sciences, Northern Illinois University, Dekalb, IL, USA.
Research square
|November 24, 2025
概括
酸 (TA) 改变了蚊子肠道细菌,减少了微生物多样性,增加了Aedes aegypti幼虫的死亡率. 这表明TA可以通过破坏它们的肠道微生物群来成为蚊子幼虫的潜在生物控制剂.
科学领域:
- 微生物学 微生物学
- 生态生态学 生态生态学
- 载体生物学 载体生物学
背景情况:
- 酸 (TA) 是一种植物性多,在蚊子繁殖场所发现.
- 尽管TA对蚊子发育和免疫的重要性,但TA对蚊子肠道微生物群的影响在很大程度上是未知的.
研究的目的:
- 研究TA对Aedes triseriatus肠道微生物群的影响.
- 探索TA作为蚊子生物控制策略的潜力.
主要方法:
- 对Aedes triseriatus中肠和养殖水中的细菌群落进行比较分析. 有或没有TA.
- 评估幼虫和成年蚊子的死亡率.
- 微生物多样性分析 (Chao 1丰富度,香农多样性).
- 遗传学分析和PICRUSt的功能预测.
主要成果:
- 补充TA使幼虫死亡率增加了50%,而卡纳米辛则增加了75%.
- 在蚊子肠道中,TA显著降低了微生物的丰富性和多样性,但在水或叶子样本中没有.
- TA改变了微生物群落结构,增加了Pseudomonadota和减少了Bacteroidota,并丰富了Pseudomonas.
- 功能预测表明微生物通过改变代谢和运输途径适应TA.
结论:
- 酸显著影响蚊子肠道微生物群的组成和多样性.
- 接触TA会导致适应性微生物反应和蚊子死亡率的增加.
- 用TA操纵蚊子微生物群是一种潜在的生物控制策略来对抗蚊子幼虫.
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