TRF2-RAP1的结构基础 EBNA1在EBV复制的EBV起源上进行招聘
Paul Lieberman1, Samantha Sustek1, Troy Messick1
1The Wistar Institute.
Research square
|November 24, 2025
概括
爱斯坦-巴尔核抗原1 (EBNA1) 使用独特的酸性补丁结合TRF2-Rap1,对爱斯坦-巴尔病毒 (EBV) DNA复制至关重要. 这种相互作用对于感染细胞中病毒发作维护至关重要.
科学领域:
- 分子生物学分子生物学
- 病毒学 病毒学
- 结构生物学 结构生物学
背景情况:
- 爱斯坦-巴尔核抗原1 (EBNA1) 对于爱斯坦-巴尔病毒 (EBV) 的DNA复制和环节性维护至关重要.
- EBV (oriP) 的最小复制起源,特别是1⁄2DS,通过非amer元素招募端粒蛋白TRF2和Rap1,但相互作用机制尚不清楚.
研究的目的:
- 阐明涉及EBNA1,TRF2和Rap1在EBV或P最小复制单位的宿主因素参与的结构基础.
- 了解EBNA1上独特的酸性补丁在这种复杂的形成中的作用及其对病毒复制的影响.
主要方法:
- 低温电子显微镜的使用方法
- 零长度交叉连接质谱法
- 在AlphaFold3建模中,
- 生物化学结合测定试验
- 变异性研究的研究.
主要成果:
- 一个动态复合的EBNA1,TRF2和Rap1被定义在1⁄2DS.
- TRF2同体化域 (TRFH) 与EBNA1上的独特的酸性补丁相互作用,与其DNA结合部位不同.
- 突变这种酸性补丁破坏了TRFH结合,并取消了ORIP依赖的等离子体复制.
结论:
- 在EBNA1上的一块未经表征的酸性补丁作为TRF2-RAP1的对接表面,对于协调这些蛋白质在ORIP至关重要.
- 这些发现为EBV复制机制和与端粒DNA复制过程的潜在联系提供了新的见解.
关键词:
化EMEM 在冷EMEM.在EBNA1中,EBNA1是指EBNA1.这是EBV的EBV.在庇护所里面.在TERF2中,TERF2是TERF2.在TERF2IP中,我们可以使用TERF2IP.在 TRF2 中,TRF2 是 TRF2.在 hRAP1 程序中使用 hRAP1.这就是为什么它是PORP.更多相关视频
10:27Spatio-Temporal Manipulation of Small GTPase Activity at Subcellular Level and on Timescale of Seconds in Living Cells
Published on: March 9, 2012
11.2K
09:58Mapping the Structure-Function Relationships of Disordered Oncogenic Transcription Factors Using Transcriptomic Analysis
Published on: June 27, 2020
3.1K
相关概念视频
Transcription Initiation
20.0K
Initiation is the first step of transcription in eukaryotes. Prokaryotic RNA Polymerase (RNAP) can bind to the template DNA and start transcribing. On the other hand, transcription in eukaryotes requires additional proteins, called transcription factors, to first bind to the promoter region in the DNA template. This binding helps recruit the specific RNAP that can assemble on the DNA and start transcription.
The promoters and enhancers and their accessory proteins allow tight regulation of...
The promoters and enhancers and their accessory proteins allow tight regulation of...
20.0K
Rab Proteins
4.9K
Rab proteins constitute the largest family of monomeric GTPases, of which 70 members are present in humans. Rab proteins and their effectors regulate consecutive stages of vesicle transport such as vesicle transport, docking, and fusion to the correct recipient membrane.
Rab proteins switch between a cytosolic, GDP-bound inactive state and a membrane-anchored, GTP-bound active state. By themselves, Rabs show slow rates of GDP/GTP exchange and GTP hydrolysis. Thus, Rab proteins are considered...
Rab proteins switch between a cytosolic, GDP-bound inactive state and a membrane-anchored, GTP-bound active state. By themselves, Rabs show slow rates of GDP/GTP exchange and GTP hydrolysis. Thus, Rab proteins are considered...
4.9K
LTR Retrotransposons
19.4K
LTR retrotransposons are class I transposable elements with long terminal repeats flanking an internal coding region. These elements are less abundant in mammals compared to other class I transposable elements. About 8 percent of human genomic DNA comprises LTR retrotransposons. Some of the common examples of LTR retrotransposons are Ty elements in yeast and Copia elements in Drosophila.
The internal coding region of LTR retrotransposons and their mechanism of transposition closely resembles a...
The internal coding region of LTR retrotransposons and their mechanism of transposition closely resembles a...
19.4K
Eukaryotic RNA Polymerases
26.6K
RNA Polymerase (RNAP) is conserved in all animals, with bacterial, archaeal, and eukaryotic RNAPs sharing significant sequence, structural, and functional similarities. Among the three eukaryotic RNAPs, RNA Polymerase II is most similar to bacterial RNAP in terms of both structural organization and folding topologies of the enzyme subunits. However, these similarities are not reflected in their mechanism of action.
All three eukaryotic RNAPs require specific transcription factors, of which the...
All three eukaryotic RNAPs require specific transcription factors, of which the...
26.6K
Rab Cascades
3.4K
Rab GTPases act in a regulated cascade during membrane fusion, helping the lipid bilayers mix. The Rab family of proteins are active when bound to GTP, and inactive when bound to GDP. Hence, they act as guanine nucleotide-dependent molecular switches. Rab-GTP recognizes and binds to long or short-range tethering proteins to capture the target vesicle. These tethers coordinate with SNAREs on the vesicle and the target membrane to assemble the trans SNARE complex that locks the mixing bilayers.
3.4K
Transcription Elongation Factors
13.2K
Transcription elongation is a dynamic process that alters depending upon the sequence heterogeneity of the DNA being transcribed. Hence, it is not surprising that the elongation complex's composition also varies along the way while transcribing a gene.
The transcription elongation is regulated via pausing of RNA polymerase on several occasions during transcription. In bacteria, these halts are necessary because the transcription of DNA into mRNA is coupled to the translation of that mRNA...
The transcription elongation is regulated via pausing of RNA polymerase on several occasions during transcription. In bacteria, these halts are necessary because the transcription of DNA into mRNA is coupled to the translation of that mRNA...
13.2K
