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下一代测序定义了捐赠的人类牛奶细菌微生物群的组成

Jurjana Novoselac¹, Andreja Leboš Pavunc², Nina Čuljak²

¹Clinical Department of Transfusion Medicine and Transplantation Biology, University Hospital Centre Zagreb, Zagreb, Croatia.

Pediatric gastroenterology, hepatology & nutrition

|November 24, 2025

概括

捐赠人乳 (DHM) 中的细菌微生物群各不相同. 储存容器和婴儿养方法等因素影响其组成和多样性,影响婴儿健康.

科学领域:

微生物学微生物学
人类牛奶研究研究
婴儿健康婴儿健康

背景情况:

人母乳微生物群在婴儿的健康和发育中起着至关重要的作用.
捐赠母乳 (DHM) 是无法从自己的母亲那里获得母乳的婴儿的重要资源.
了解DHM的微生物成分对于确保其安全性和有效性至关重要.

研究的目的:

调查捐赠人乳 (DHM) 中的细菌微生物群组成和多样性.
确定影响DHM微生物群的潜在因素,包括捐赠者,婴儿和与牛奶相关的变量.

主要方法:

在分娩后六个月内从单个捐赠者获得88个原始DHM样本的分析.
使用Illumina MiSeq.进行细菌16S核糖体RNA基因测序 (V1-V3区域)
对捐赠者,婴儿和牛奶相关数据进行统计分析,以确定影响因素.

主要成果:

葡萄球菌是最丰富的细菌属.
乳酸菌在所有样本中都存在,其丰度因储存容器类型 (瓶子与袋子) 显著不同.
在70%的样本中发现了Bifidobacterium. 在纯母乳养婴儿的牛奶和手工取样本中观察到更高的α多样性.

结论:

捐赠者,婴儿和牛奶的特征显著影响细菌微生物群的组成和多样性.
这项研究强调了在DHM加工和利用中考虑这些因素的重要性.
这些发现有助于理解聚合的DHM微生物群,这是以前高通量测序研究较少探索的领域.

关键词:

细菌细菌是一种细菌.养方法养方法高通量核酸测序的测序微生物群中的微生物群牛奶银行的牛奶银行牛奶,人类的牛奶核糖核酸,核糖体,16S,16S,16S,16S,16S,16S.

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