电子显微镜可视化人类血,血清和唾液中含有细胞外囊泡的无细胞线粒体DNA
Alexandra Volos1, Soah Grace Franklin1, Jeremy Michelson1
1Department of Psychiatry, Division of Behavioral Medicine, Columbia University Irving Medical Center, New York, NY 10032, United States.
medRxiv : the preprint server for health sciences
|November 24, 2025
概括
在人类生物流体中发现的无细胞线粒体DNA (cf-mtDNA) 和细胞外线粒体 (ex-Mito). 这项研究可视化了这些粒子,表明cf-mtDNA可能被包装在前Mito内,而不是自由漂浮.
科学领域:
- 细胞生物学 细胞生物学
- 生物化学 生物化学
- 线粒体生物学 线粒体生物学
背景情况:
- 人类生物流体含有无细胞线粒体DNA (cf-mtDNA) 和细胞外线粒体 (ex-Mito).
- 这些细胞外成分的起源,目的地,调节和功能仍然不清楚.
- 了解cf-mtDNA和ex-Mito生物学对于各种生理和病理过程至关重要.
研究的目的:
- 用电子显微镜鉴定人类生物流体中的循环颗粒,包括前Mito和细胞外囊泡 (EVs).
- 在血,血清和唾液中量化无细胞线粒体DNA (cf-mtDNA).
- 调查cf-mtDNA水平与前Mito-like粒子存在之间的关系.
主要方法:
- 收集了十个健康个体的血,血清和唾液.
- 通过离心和尺寸排除色谱分离的细胞外颗粒.
- 执行传输电子显微镜用于粒子成像和形态分析.
- 在每个生物流体中的量化cf-mtDNA度.
主要成果:
- 识别了循环的双膜细胞外粒子,与线粒体超结构一致,在人类生物流体中,与EV和其他粒子类型一起.
- 观察到较高的血cf-mtDNA度与增加的双膜,前米托样粒子数量相关的趋势.
- 在正常情况下,大多数cf-mtDNA可能不会以自由的,裸体的形式存在.
结论:
- 在人体血,血清和唾液中存在循环的细胞外线粒体 (ex-Mito) 类粒子.
- 这些发现挑战了主要是自由cf-mtDNA的概念,在细胞间通信或线粒体转移中起到支持作用.
- 这项研究提供了循环颗粒的基础目录,并为未来关于ex-Mito和cf-mtDNA量化研究提供了信息.
关键词:
没有细胞的材料是无细胞的.循环的循环循环的循环人类 人类 人类 人类 人类 人类 人类图像存储库 图像存储库是一个图像存储库.影像成像技术 影像成像技术显微镜 显微镜是指使用显微镜.线粒体中的线粒体.研究设计研究设计囊中的泡更多相关视频
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