通过元基因组下一代测序来探索中枢神经系统感染中的微生物群的光谱
Jun-Mei Wang1,2, Yu-Ying Pan1,2, Jian-Chen Hong3
1Department of Neurology, Fujian Institute of Neurology, the First Affiliated Hospital, Fujian Medical University, Fuzhou, 350005, People's Republic of China.
Infection and drug resistance
|November 24, 2025
概括
下一代元基因组测序 (mNGS) 在中枢神经系统 (CNS) 感染的脑脊液 (CSF) 中识别了各种病原体. 这种先进的技术有助于了解微生物特征,并指导中枢神经系统感染的治疗决策.
科学领域:
- 微生物学 微生物学
- 传染性疾病 传染性疾病
- 基因组学就是基因组学.
背景情况:
- 中枢神经系统 (CNS) 感染带来了诊断上的挑战.
- 了解中枢神经系统感染中微生物病原体的全谱对于有效治疗至关重要.
- 下一代元基因组测序 (mNGS) 为微生物识别提供了一种全面的方法.
研究的目的:
- 通过使用CSF mNGS来阐明中枢神经系统感染患者的微生物群谱.
- 识别导致中枢神经系统感染的常见和罕见病原体.
- 评估mNGS在诊断中枢神经系统感染中的有用性.
主要方法:
- 对大脑脊髓液 (CSF) 的转基因下一代测序 (mNGS) 报告和临床数据的回顾性分析.
- 该研究从2019年9月到2023年11月包括264名疑似中枢神经系统感染的患者.
- 使用诊断标准来确认中枢神经系统感染,将其分为细菌,结核,真菌和病毒类型.
主要成果:
- 在被诊断的145种中枢神经系统感染中,病毒性感染 (44.8%) 和Mycobacterium结核病 (20.7%) 是最常见的.
- 阳性mNGS检测率为46.2%,在不同类型的感染中差异很大 (p < 0.001).
- 确定的主要病原体包括Cryptococcus neoformans (23.9%),Mycobacterium tuberculosis (14.9%) 和爱斯坦-巴尔病毒 (13.4%). 常见的背景微生物是Cutibacterium acnes (58.6%),Moraxella osloensis (29.0%) 和Malassezia restricta (26.2%),这些微生物是最常见的.
结论:
- 高通量mNGS有效地揭示了中枢神经系统感染患者的CSF中的微生物组成.
- 这种方法提高了对中枢神经系统感染中致病原体的理解.
- mNGS可以帮助临床医生为中枢神经系统感染做出明智的治疗决策.
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