对于单细胞亚细胞结构表型抗菌素敏感性测试的时间缩短深度学习
Wenwen Jing1, Tianran Zhang1, Xi Chen2
1Key Laboratory of Medical Molecular Virology, MOE & NHC, Shanghai Institute of Infectious Disease and Biosecurity, School of Basic Medical Sciences, Fudan University, Shanghai, 200032, China.
Analytical chemistry
|November 24, 2025
概括
一种新的快速表型抗微生物敏感性测试 (AST) 方法使用显微镜和深度学习在20分钟内提供结果. 这种方法可以准确地识别抗菌素耐药性 (AMR),并揭示单细胞异质性,有助于及时做出临床决定.
科学领域:
- 微生物学与传染病的研究
- 生物技术和医学成像技术
- 医疗保健中的人工智能
背景情况:
- 抗菌素耐药性 (AMR) 构成了全球健康的重大威胁,增加了疾病的严重程度和医疗保健成本.
- 传统的抗微生物敏感性测试 (AST) 方法是缓慢的 (24小时至几天) 并依赖于培养.
- 基因型检测仅限于已知的耐药性基因,不能检测出新型变异.
研究的目的:
- 开发一个快速,准确的表型AST平台,以克服当前方法的局限性.
- 利用结构化照明显微镜 (SIM) 和深度学习进行亚细胞细菌表型评估.
- 为了使AST更快,更有效地解决及时的临床决策和AMR制.
主要方法:
- 开发一个快速的表型AST平台,集成SIM成像和深度学习.
- 七个深度学习架构 (ResNet-50,C3D,DenseNet-121等) 的培训 在细菌图像数据集上.
- 应用单细胞分析在最小抑制度 (MIC) 附近,以评估细菌反应.
主要成果:
- 对于大肠杆菌,ResNet-50在不到20分钟的时间内实现了AST结果的87%准确性,对于M. smegmatis,在4小时内,对于BCG. smegmatis,在15小时内.
- 深度学习平台表现出与传统的AST测试有很强的一致性.
- 单细胞分析揭示了细菌对抗生素反应的异质性,掩盖了人口水平的MIC.
结论:
- 开发的方法能够在没有培养要求的情况下快速,亚细胞水平的表型AST.
- 这个平台准确地评估了抗生素的有效性,并揭示了单细胞异质性,这对于理解耐药性至关重要.
- 这些发现支持及时的临床决策,并提供了一种打击AMR传播的工具.
更多相关视频
05:57Live-Cell Fluorescence Microscopy to Investigate Subcellular Protein Localization and Cell Morphology Changes in Bacteria
Published on: November 23, 2019
7.7K
07:44Time-Lapse Epifluorescence Microscopy Imaging of Pseudomonas aeruginosa and Staphylococcus aureus Heterogeneous Phenotypes
Published on: February 14, 2025
1.5K
相关概念视频
01:26Differential Staining Technique
Differential staining is an essential microbiological technique that exploits variations in cell wall structures to classify and identify microorganisms. It facilitates the distinction of bacteria, aiding in diagnostic and research applications. Two of the most widely used differential staining methods are Gram staining and acid-fast staining, both of which rely on the chemical and structural differences in bacterial cell walls.Gram Staining TechniqueGram staining differentiates bacteria by...
01:13Special Staining Techniques
Specialized staining techniques play a vital role in microbiology by enabling the visualization of specific bacterial structures that remain undetectable with standard microscopy methods. These techniques not only enhance the structural visualization of bacterial cells but also provide critical insights into their pathogenicity and classification. Additionally, they support diagnostic and research endeavors in microbiology by identifying key bacterial features.Capsule Staining for Virulence...
01:25Rapid Identification of Pathogens
MALDI-TOF MS has transformed clinical microbiology by offering a rapid and reliable method for pathogen identification. The traditional approach to microbial identification typically involves time-consuming culture techniques and biochemical tests, which can delay the initiation of appropriate antimicrobial therapy. MALDI-TOF MS avoids these delays by using characteristic ribosomal protein mass patterns of microbial cells, enabling accurate species-level identification within minutes.Principle...