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通过BSA复合,通过本地乳酸细菌产生的生物活性叶酸的稳定

Fenny Amilia Mahara¹, Lala Adetia Marlina², Moch Rozak Sofwatillah Apriliadi³

¹Research Center for Food Technology and Processing, National Research and Innovation Agency (BRIN), Jl. Jogja-Wonosari KM 31.5, Gunungkidul, 55861, Yogyakarta, Indonesia.

World journal of microbiology & biotechnology

|November 24, 2025

概括

研究人员发现了一种有前途的乳酸细菌,Lactiplantibacillus plantarum RAL25,来自印度尼西亚的开菲尔谷物,用于生产5-甲基四基酸盐 (5-MTHF). 这项研究还研究了使用牛血清白蛋白 (BSA) 稳定5-MTHF.

科学领域:

微生物学微生物学
生物化学生物化学
食品科学食品科学食品科学

背景情况:

叶酸缺乏症是一个全球性的健康问题,原因是不稳定和稀缺的天然叶酸形式.
微生物的减少叶酸的生产,如四基叶酸 (THF) 和5-甲基四基叶酸 (5-MTHF) 为生物可用性提供了一个可持续的解决方案.
生物活性叶酸盐的稳定对于其在食品和补充剂中的应用至关重要.

研究的目的:

选印度尼西亚开衍生乳酸细菌 (LAB) 进行细胞外5-甲基四基酸盐 (5-MTHF) 生产.
通过使用牛血清白蛋白 (BSA) 来评估5-MTHF基于蛋白质的稳定潜力.
为了提高稳定性,研究5-MTHF和BSA之间的相互作用.

主要方法:

选29个LAB隔离物从开菲尔谷物中的5-MTHF和THF生物合成.
通过最强的分离物产生的5-MTHF的部分净化和表征.
谱分析和分子模拟以评估5-MTHF和BSA相互作用.

主要成果:

乳植物菌植物植物RAL25被确定为5-MTHF (20.94±8.36μg/mL) 的最大生产者.
光谱数据证实了产生的5-MTHF的身份及其与BSA的相互作用.
分子模拟表明,5-MTHF和BSA之间通过结和疏水相互作用形成稳定的复合物.

结论:

L. plantarum RAL25是天然5-MTHF生产的有希望的来源.
BSA复杂化为稳定减少的叶酸盐提供了机制基础.
对于功能性食品的应用,需要进一步研究稳定性和生物可用性.

关键词:

5甲基四基酸盐 (5-MTHF) 是一种生物生产是一种生物生产.牛血清专蛋白 (BSA) 是一种复杂化的复杂化乳酸细菌 (LAB) 是一种稳定的稳定性稳定的稳定性

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