葡萄葡萄蛋白Src2在Lasiodiplodia theobromae感染期间调解植物疾病抵抗力
Junbo Peng1, Qikai Xing1, Wei Zhang1
1Beijing Key Laboratory of Environment Friendly Management on Diseases and Pests of North China Fruits, Institute of Plant Protection, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China.
Plant physiology
|November 24, 2025
概括
lysine motif (LysM) 作用因子是关键的毒性因子. 这项研究揭示了Lasiodiplodia theobromae是如何形成的.
科学领域:
- 植物病原体相互作用
- 分子植物病理学 分子植物病理学
- 分子植物微生物相互作用.
背景情况:
- 素基因 (LysM) 作用因子是许多植物病原体中关键的毒性因素.
- 对于LysM效应器的功能,其确切的机制仍然在很大程度上是未知的.
- 拉西奥迪普洛迪亚 (Lasiodiplodia theobromae) 是一种机会性植物病原体,使用LysM效应器.
研究的目的:
- 研究来自Lasiodiplodia theobromae的LysM效应器LtLysM2的作用和机制.
- 为了阐明LtLysM2和葡萄葡萄蛋白在植物防御中的相互作用.
- 了解LtLysM2在植物感染期间启动的信号通路.
主要方法:
- 在L. theobromae中的LysM效应器LtLysM2的识别和表征.
- 基结合测试以确定LtLysM2与基寡糖的相互作用.
- 酵母两杂交试验和共免疫沉以研究蛋白质-蛋白质相互作用.
- 在Nicotiana benthamiana的子宫外表达,以评估VvSrc2在植物耐药性的作用.
- 同焦显微镜分析VvSrc2.2的亚细胞局部化.
主要成果:
- LtLysM2被确定为L. theobromae中的毒性因子,能够结合素并抑制植物免疫力.
- 葡萄葡萄蛋白VvSrc2与LtLysM2相互作用,促进其核积累.
- 宫外表达的VvSrc2赋予增强的抵抗力L. theobromae 在N. benthamiana.
- VvSrc2与核RNA结合蛋白VvUbp1相互作用,调解下游信号传输.
结论:
- LtLysM2通过抑制基触发的免疫力,有助于L. theobromae的毒性.
- VvSrc2充当关键的宿主调解者,与病原体效应物和下游核蛋白相互作用.
- 这项研究揭示了一种新的植物防御途径,涉及VvSrc2和VvUbp1对L. theobromae感染的反应.
相关概念视频
01:25Cell Signaling in Plants
6.1K
Plant cells communicate to coordinate their cycle of growth, flowering and fruiting, and activities in roots, shoots, and leaves in response to the changing environmental conditions. Plant signaling is distinct from animal signaling. Plants primarily utilize enzyme-linked receptors, whereas the largest class of cell-surface receptors in animals are G-protein coupled receptors (GPCRs). Unlike animals, receptor tyrosine kinases are rare in plants. Instead, plants have a diverse class of...
6.1K
02:43Plant Cell Wall
59.9K
The plant cell wall gives plant cells shape, support, and protection. As a cell matures, its cell wall specializes according to the cell type. For example, the parenchyma cells of leaves possess only a thin, primary cell wall.
59.9K
02:26Defenses Against Pathogens and Herbivores
29.4K
Plants present a rich source of nutrients for many organisms, making it a target for herbivores and infectious agents. Plants, though lacking a proper immune system, have developed an array of constitutive and inducible defenses to fend off these attacks.
29.4K
02:22Introduction to Plant Diversity
48.4K
From Water to Land
48.4K
01:17Gene Regulation During Sporulation
420
Sporulation is a complex developmental process that allows certain Gram-positive bacteria, such as Bacillus subtilis and Clostridium species, to survive extreme environmental conditions. This process is tightly regulated by a series of signaling cascades and transcriptional controls, ensuring the formation of a highly resistant endospore.Sporulation is triggered by unfavorable conditions, such as nutrient depletion, and is governed by a phosphorelay system. One of the sensor kinases, such as...
420