细胞分裂周期5对于在本地化过程中在FLC上发挥非编码RNA和PRC2的抑制功能很重要
Xin Xin1, Jinlong Li2, Shu Wang1
1State Key Laboratory of Wheat Improvement, College of Life Sciences, Shandong Agricultural University, Tai'an, 271018, China.
The Plant journal : for cell and molecular biology
|November 24, 2025
概括
R2R3-MYB转录因子CDC5对于冬季诱导的开花至关重要. 疾病预防控制中心5链接非编码RNA转录和表观遗传沉默的花C (FLC) 在寒冷暴露期间.
科学领域:
- 植物生物学 植物生物学
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
- 分子生物学分子生物学
背景情况:
- 花开时间由FLOWERING LOCUS C (FLC) 调节,这是一个关键压缩器.
- 弗纳化 (暴露于寒冷) 通过非编码RNA (COOLAIR/COLDAIR) 和多抑制复合体2 (PRC2) 的表观遗传沉默来抑制FLC.
- 在寒冷期间非编码RNA转录和PRC2功能的整合仍然不清楚.
研究的目的:
- 为了确定对本地化反应的开花的调节者.
- 阐明了结合非编码RNA转录和PRC2功能在冷时FLC调节中的机制.
主要方法:
- 对cdc5-2突变的遗传分析.
- 染色体免疫沉 (ChIP) 试验用于评估FLC染色体修饰和RNA聚合酶II丰富.
- 对COOLAIR和COLDAIR非编码RNA转录的分析.
- 同免疫沉研究CDC5与PRC2组件的相互作用.
主要成果:
- 在cdc5-2突变体中,早期的开花正常,但在本地化后的开花延迟.
- 在寒冷条件下,cdc5-2突变体在FLC抑制,非编码RNA转录和H3K27me3沉积方面表现出缺陷.
- CDC5结合FLC染色体,影响RNA聚合酶II的丰富和COOLAIR/COLDAIR的转录.
- CDC5在物理上与PRC2相互作用,作为FLC中H3K27me3建立的辅因子.
结论:
- CDC5 是一种关键的调节器,对依赖于本地化的开花产生影响.
- CDC5协调非编码RNA转录与PRC2-介导的表观遗传沉默在FLC.
- CDC5跨越了FLC的转录和表观遗传控制,调解了冬季诱导的开花调节.
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