DYW类型的三重复蛋白DEK618与ZmMORFs相互作用,调节线粒体RNA编辑和玉米内核发育
Song Zhang1, Zheyuan Wang2, Zilong Wang3
1State Key Laboratory of Crop Gene Resources and Breeding, National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China; Beijing Innovation Center for Crop Seed Technology (MOA), College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, China.
Plant science : an international journal of experimental plant biology
|November 24, 2025
概括
研究人员确定了DEK618,这是一种对玉米线粒体中C-to-URNA编辑至关重要的新型蛋白质. 它的损失导致内核发育缺陷,并影响线粒体功能和复杂III组装.
科学领域:
- 植物分子生物学 植物分子生物学
- 线粒体遗传学线粒体遗传学
- 基因组RNA的修饰是RNA的修饰.
背景情况:
- 丁-丁 (C-to-U) 编辑对于植物线粒体RNA功能至关重要.
- 五重复蛋白 (PPR) 蛋白质,特别是DYW型 (DYW-PPR),是RNA编辑的关键调节者.
- 在玉米中,许多DYW-PPR蛋白的作用在很大程度上是未知的.
研究的目的:
- 为了描述一种新的玉米DYW-PPR蛋白的功能,DEK618.
- 研究DEK618对C-to-URNA编辑和线粒体完整性的影响.
- 为了确定涉及RNA编辑的潜在蛋白质相互作用.
主要方法:
- 一种玉米突变的遗传分析,玉米内核发育缺陷和矮体.
- 关于"Dek618"基因及其蛋白质产物的分子特征.
- 在多个线粒体RNA位点对C-to-U编辑效率的分析.
- 对线粒体形态和复杂III组合的研究.
- 同免疫沉测试以确定蛋白质相互作用.
主要成果:
- `Dek618`中的突变导致了缺陷的核发育和矮体,与异常的线粒体形态有关.
- DEK618功能丧失导致88个C-to-U站点的编辑效率发生了变化.
- 在关键部位 (cob-298,ccmC-184,ccmB-428,ccmFC-1219) 观察到完全失去编辑,影响线粒体复合物III.
- DEK618与ZmMORF1和ZmMORF8.8进行物理相互作用.
结论:
- DEK618是一种新型的DYW-PPR蛋白质,对于玉米线粒体中的C-to-URNA编辑至关重要.
- DEK618在维护线粒体功能方面发挥着关键作用,可能是通过其参与复杂III组装.
- DEK618与ZmMORF1和ZmMORF8一起作为蛋白质复合体的一部分,以促进RNA编辑.
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