老动物的创伤后关节炎与与关节重塑相关的脑部变化有关
Jacob L Griffith1, Pedro A Valdes-Hernandez2, Taylor D Yeater1
1J. Crayton Pruitt Family Department of Biomedical Engineering, University of Florida, Gainesville, FL, USA.
The journal of pain
|November 24, 2025
概括
骨关节炎 (OA) 导致关节损伤,以及大脑重塑,影响疼痛感知. 这项对老老鼠的研究表明,手术膝盖损伤导致脑部参与疼痛处理的区域发生变化.
科学领域:
- 神经科学是一个神经科学.
- 整形外科 整形外科 整形外科
- 疼痛研究 疼痛研究
背景情况:
- 临床前关节炎 (OA) 模型主要评估关节变化和疼痛行为.
- 对OA对大脑重塑和中央疼痛处理的影响仍然不太清楚.
- 老年动物模型提供了关于OA发展和相关神经系统变化的见解.
研究的目的:
- 用手术后创伤性关节炎模型研究老老鼠的关节和大脑重塑.
- 分析触觉灵敏度的变化,神经成像 (灰质体积,BOLD信号,功能连接性) 和关节组织学.
- 确定骨质炎的发展是否与受影响关节以外的中枢神经系统变化有关.
主要方法:
- 年龄较大的雄性和雌性费舍尔344只老鼠接受了假手术 (皮肤切口) 或中间侧带和中间阴囊切口 (MCLT+MMT) 来建模OA.
- 在手术后的多个时间点评估触觉灵敏度.
- 神经成像 (MRI) 进行了手术前和手术后6周和14周,重点关注大脑结构和功能. 组织学分析检查了关节组织.
主要成果:
- 组织学证实,与虚假对照组相比,MCLT+MMT组的软骨损失和骨损伤显著.
- 尽管两组的触觉灵敏度变化相似,神经成像显示了MCLT+MMT和两性假装组之间的显著差异.
- 参与疼痛传输,调制和情绪处理的大脑区域 (脑膜,体感皮质,水管灰色,条体,海马体,前额皮质,杏仁体) 显示灰色物质体积,BOLD信号和功能连接发生变化.
结论:
- 在老老鼠中,手术诱导关节炎导致大脑重塑,即使总体触觉灵敏度的变化在不同组之间是相似的.
- 这些大脑变化发生在对于疼痛处理的感觉,情感和调节方面至关重要的区域.
- 这项研究提供了与骨关节炎相关的中枢神经系统变化的证据,突出了关节之外的影响,并为未来研究验证了该模型.
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