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1H,C和N的脊柱共振赋予从Lentinus similis获得的Lytic多糖胺单氧化酶LsAA9A

Piera Wiesinger¹, Mats Sandgren¹, Gustav Nestor²

¹Department of Molecular Sciences, Swedish University of Agricultural Sciences, Uppsala, Sweden.

Biomolecular NMR assignments

|November 25, 2025

概括

性多糖体单氧化酶 (LPMOs) 是一种分解碳水化合物的铜酶. 本研究详细介绍了来自Lentinus similis的LPMO LsAA9A的骨干共振分配和二次结构预测.

科学领域:

生物化学生物化学
结构生物学结构生物学
酶学是一种酶学.

背景情况:

性多糖胺单氧化酶 (LPMOs) 是铜结合酶,对于碳水化合物降解至关重要.
来自Lentinus similis的LPMO LsAA9A准纤维素和纤维聚合物,在C4位置裂解糖键.
了解LPMO的结构和功能是生物质转化应用的关键.

研究的目的:

为了确定LPMO LsAA9A.的apo形式的骨干共振赋值.
使用NMR数据预测LsAA9A的二次结构,并将其与现有的晶体结构进行比较.
为生物技术应用中LPMOs的结构性特征作出贡献.

主要方法:

在大肠杆菌中LsAA9A的重组表达具有13C和15N同位素标记.
核磁共振 (NMR) 光谱用于骨干共振分配 (1H, 13C, 15N).
基于NMR化学转移的二次结构预测的TALOS-N软件.

主要成果:

为apo-LsAA9A实现了完整的1H,13C和15N骨干共振分配.
二次结构预测显示与已知的晶体结构普遍一致,螺旋和板含量存在小差异.
LsAA9A表现出特有的LPMO结构图案,具有核心β-三明治.

结论:

核磁共振赋值为进一步对LsAA9A.的结构和功能研究提供了基础.
核磁共振预测和晶体结构之间的差异凸显了LPMOs的动态性质或表达条件的潜在差异.
这项研究证实了LPMOs的结构特征,包括β-三明治核心.

关键词:

这是LsAA9AA.蜂性聚合物是细胞性聚合物.纤维素纤维素是一种纤维素.性多糖胺单氧化酶 (LPMO) 是一种这就是NMR的NMR.

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